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蓝桉树叶和果实的不同溶剂提取物对不同细菌和真菌生长的影响。

Impact of different solvent extracts from leaves and fruits of Eucalyptus globulus on growth of different bacteria and fungi.

作者信息

Bakht Jehan, Farid Saman, Iqbal Arshad, Shafi Mohammad

机构信息

Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar KPK, Pakistan.

Department of Botany, Islamia College, Peshawar, KPK Pakistan.

出版信息

Pak J Pharm Sci. 2018 Sep;31(5):1845-1852.

Abstract

The present research investigates the antimicrobial activities of the samples extracted from the leaves and fruits of Eucalyptus globulus through disc diffusion susceptibility assay using 1, 2 and 3mg disc-1 concentrations. Different extracted samples from the leaves and fruits of Eucalyptus globulus exhibited different degrees of antimicrobial. The data indicated that n-butanol and ethyl acetate extracted fraction of both the leaves and fruits inhibited the growth of all microorganisms at all the tested concentrations. Aqueous extracted sample of the leaves inhibited the growth of Candida albicans while the same fraction from the fruits showed no activity against Bacillus subtilis at any concentration. N-hexane extracted samples of the leaves inhibited the growth of Bacillus subtilis, E. coli and Pseudomonas aeruginosa at the tested concentrations while no activity was recorded against Klebsiella pneumonia, Candida albicans and Stephylococcus aureus. N-butanol extracted samples from the leaves and fruits showed activity against Pseudomonas aeruginosa at the tested concentrations. In case of leaves, the most susceptible bacteria was Bacillus subtilis (gram positive) and Stephylococcus aureus (gram positive) was the most resistant one. In case of fruits the most susceptible bacteria was Stephylococcus aureus (gram positive) and E. coli (Gram negative) was the most resistant one.

摘要

本研究通过纸片扩散药敏试验,使用每片1mg、2mg和3mg的浓度,对蓝桉树叶和果实中提取的样品的抗菌活性进行了研究。从蓝桉树叶和果实中提取的不同样品表现出不同程度的抗菌活性。数据表明,叶和果实的正丁醇和乙酸乙酯提取物在所有测试浓度下均能抑制所有微生物的生长。叶的水提取物能抑制白色念珠菌的生长,而果实的相同提取物在任何浓度下对枯草芽孢杆菌均无活性。叶的正己烷提取物在测试浓度下能抑制枯草芽孢杆菌、大肠杆菌和铜绿假单胞菌的生长,而对肺炎克雷伯菌、白色念珠菌和金黄色葡萄球菌无活性。叶和果实的正丁醇提取物在测试浓度下对铜绿假单胞菌有活性。就叶而言,最敏感的细菌是枯草芽孢杆菌(革兰氏阳性),最耐药的是金黄色葡萄球菌(革兰氏阳性)。就果实而言,最敏感的细菌是金黄色葡萄球菌(革兰氏阳性),最耐药的是大肠杆菌(革兰氏阴性)。

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