Department of Biochemistry and Molecular Biology, Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu, China.
School of Life Sciences, China Pharmaceutical University, Nanjing, Jiangsu, China.
FASEB J. 2018 Mar;32(3):1139-1153. doi: 10.1096/fj.201700741RR. Epub 2018 Jan 3.
Gut dysbiosis is believed to play a critical role in the pathogenesis of metabolic diseases, including obesity. Ophiopogonin D (OP-D), a steroidal glycoside and an active component extracted from the root of the plant Ophiopogon japonicus (also known as radix ophiopogonis), can regulate multiple physiologic processes. Here we show that OP-D administration reduces body weight, hyperglycemia, hyperlipidemia, and insulin resistance in male mice fed a high-fat diet (HFD). Pyrosequencing of the V4 regions of 16S rRNA genes in mouse feces revealed a deviation of the gut microbiota in response to OP-D treatment. In particular, the decreased Firmicutes-to- Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels indicated that OP-D reversed HFD-induced gut dysbiosis. More importantly, the effects of OP-D on modulation of obesity and microbiota were transferable via horizontal feces transfer from OP-D-treated mice to HFD-fed mice. Taken together, our results suggest that OP-D may be used as a prebiotic agent to treat obesity-associated gut dysbiosis and metabolic syndrome.-Chen, S., Li, X., Liu, L., Liu, C., Han, X. Ophiopogonin D alleviates high-fat diet-induced metabolic syndrome and changes the structure of gut microbiota in mice.
肠道菌群失调被认为在代谢性疾病（包括肥胖症）的发病机制中起着关键作用。麦冬苷 D（OP-D）是一种甾体糖苷，也是从植物麦冬（也称为麦冬根）中提取的一种活性成分，可调节多种生理过程。在这里，我们发现给予 OP-D 可降低高脂饮食（HFD）喂养的雄性小鼠的体重、高血糖、高血脂和胰岛素抵抗。对小鼠粪便 16S rRNA 基因 V4 区进行焦磷酸测序显示，肠道微生物群对 OP-D 治疗有反应。特别是，厚壁菌门与拟杆菌门比值和携带内毒素的变形菌门水平的降低表明，OP-D 逆转了 HFD 诱导的肠道菌群失调。更重要的是，OP-D 通过将来自 OP-D 处理的小鼠的水平粪便转移到 HFD 喂养的小鼠中，对调节肥胖和微生物群的作用具有可转移性。综上所述，我们的研究结果表明，OP-D 可用作治疗肥胖相关肠道菌群失调和代谢综合征的益生元。
