• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大麻素受体 1 的药理学抑制通过 mTOR 通路刺激 nesfatin-1 的胃释放。

Pharmacological inhibition of cannabinoid receptor 1 stimulates gastric release of nesfatin-1 via the mTOR pathway.

机构信息

Grupo Fisiopatología Endocrina, Instituto de Investigación Sanitaria de Santiago de Compostela, 15706 Santiago de Compostela, Spain.

CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, 15782 Santiago de compostela, Spain.

出版信息

World J Gastroenterol. 2017 Sep 21;23(35):6403-6411. doi: 10.3748/wjg.v23.i35.6403.

DOI:10.3748/wjg.v23.i35.6403
PMID:29085189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5643265/
Abstract

AIM

To determine whether Nucb2/nesfatin1 production is regulated by the cannabinoid system through the intracellular mTOR pathway in the stomach.

METHODS

Sprague Dawley rats were treated with vehicle, rimonabant, rapamycin or rapamycin+rimonabant. Gastric tissue obtained from the animals was used for biochemical assays: Nucb2 mRNA measurement by real time PCR, gastric Nucb2/nesfatin protein content by western blot, and gastric explants to obtain gastric secretomes. Nucb2/nesfatin levels were measured in gastric secretomes and plasma using enzyme-linked immunosorbent assay.

RESULTS

The inhibition of cannabinoid receptor 1 (CB1) by the peripheral injection of an inverse agonist, namely rimonabant, decreases food intake and increases the gastric secretion and circulating levels of Nucb2/nesfatin-1. In addition, rimonabant treatment activates mTOR pathway in the stomach as showed by the increase in pmTOR/mTOR expression in gastric tissue obtained from rimonabant treated animals. These effects were confirmed by the use of a CB1 antagonist, AM281. When the intracellular pathway mTOR/S6k was inactivated by chronic treatment with rapamycin, rimonabant treatment was no longer able to stimulate the gastric secretion of Nucb2/nesfatin-1.

CONCLUSION

The peripheral cannabinoid system regulates food intake through a mechanism that implies gastric production and release of Nucb2/Nesfatin-1, which is mediated by the mTOR/S6k pathway.

摘要

目的

通过胃细胞内 mTOR 通路来确定大麻素系统是否调节 Nucb2/nesfatin1 的产生。

方法

用 vehicle（对照）、rimonabant（利莫那班）、rapamycin（雷帕霉素）或 rapamycin+rimonabant 处理 Sprague Dawley 大鼠。从动物中获得胃组织，用于生化测定：实时 PCR 测量 Nucb2 mRNA，western blot 测量胃 Nucb2/nesfatin 蛋白含量，胃外植体获得胃分泌液。用酶联免疫吸附试验测量胃分泌液和血浆中的 Nucb2/nesfatin 水平。

结果

外周注射反向激动剂，即利莫那班，抑制大麻素受体 1（CB1），减少食物摄入，增加胃分泌和循环 Nucb2/nesfatin-1 水平。此外，利莫那班处理通过增加胃组织中 pmTOR/mTOR 的表达来激活胃内 mTOR 通路，从 rimonabant 处理动物中获得的胃组织。这些作用通过使用 CB1 拮抗剂 AM281 得到了证实。当慢性用 rapamycin 处理使细胞内 mTOR/S6k 途径失活时，利莫那班处理不再能够刺激 Nucb2/nesfatin-1 的胃分泌。

结论

外周大麻素系统通过涉及 mTOR/S6k 途径的机制调节食物摄入，该机制暗示胃产生和释放 Nucb2/Nesfatin-1。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/43ad589311b0/WJG-23-6403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/ed55905dbefa/WJG-23-6403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/0a17c12cf58a/WJG-23-6403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/ba2d5208ad89/WJG-23-6403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/2488a4c42aa1/WJG-23-6403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/43ad589311b0/WJG-23-6403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/ed55905dbefa/WJG-23-6403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/0a17c12cf58a/WJG-23-6403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/ba2d5208ad89/WJG-23-6403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/2488a4c42aa1/WJG-23-6403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d77/5643265/43ad589311b0/WJG-23-6403-g005.jpg

相似文献

1
Pharmacological inhibition of cannabinoid receptor 1 stimulates gastric release of nesfatin-1 via the mTOR pathway.大麻素受体 1 的药理学抑制通过 mTOR 通路刺激 nesfatin-1 的胃释放。
World J Gastroenterol. 2017 Sep 21;23(35):6403-6411. doi: 10.3748/wjg.v23.i35.6403.
2
mTOR-dependent modulation of gastric nesfatin-1/NUCB2.胃饥饿素-1/核结合蛋白2的mTOR依赖性调节
Cell Physiol Biochem. 2012;29(3-4):493-500. doi: 10.1159/000338503. Epub 2012 Apr 3.
3
Lipopolysaccharide increases gastric and circulating NUCB2/nesfatin-1 concentrations in rats.脂多糖增加大鼠胃和循环中 NUCB2/nesfatin-1 的浓度。
Peptides. 2011 Sep;32(9):1942-7. doi: 10.1016/j.peptides.2011.07.006. Epub 2011 Jul 18.
4
The gastric CB1 receptor modulates ghrelin production through the mTOR pathway to regulate food intake.胃 CB1 受体通过 mTOR 途径调节生长激素释放肽的产生，从而调节食物摄入。
PLoS One. 2013 Nov 26;8(11):e80339. doi: 10.1371/journal.pone.0080339. eCollection 2013.
5
Hypothalamic nesfatin-1/NUCB2 knockdown augments hepatic gluconeogenesis that is correlated with inhibition of mTOR-STAT3 signaling pathway in rats.下丘脑 nesfatin-1/NUCB2 敲低增强了肝糖异生，这与大鼠中 mTOR-STAT3 信号通路的抑制有关。
Diabetes. 2014 Apr;63(4):1234-47. doi: 10.2337/db13-0899. Epub 2014 Jan 29.
6
Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice.营养素在体外培养的胃生长激素释放肽瘤（MGN3-1）细胞中以及在雄性小鼠体内对核结合蛋白-2/核饱素-1有不同的调节作用。
PLoS One. 2014 Dec 15;9(12):e115102. doi: 10.1371/journal.pone.0115102. eCollection 2014.
7
Ontogenic pattern of nucleobindin-2/nesfatin-1 expression in the gastroenteropancreatic tissues and serum of Sprague Dawley rats.斯普拉格-道利大鼠胃肠胰腺组织及血清中核结合蛋白-2/核饱素-1表达的个体发生模式
Regul Pept. 2012 Apr 10;175(1-3):61-9. doi: 10.1016/j.regpep.2012.02.006. Epub 2012 Feb 28.
8
Regulation of NUCB2/nesfatin-1 production in rat's stomach and adipose tissue is dependent on age, testosterone levels and lactating status.大鼠胃和脂肪组织中NUCB2/nesfatin-1生成的调节取决于年龄、睾酮水平和泌乳状态。
Mol Cell Endocrinol. 2015 Aug 15;411:105-12. doi: 10.1016/j.mce.2015.04.016. Epub 2015 Apr 23.
9
Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa.大鼠胃泌酸黏膜内分泌细胞类型中nesfatin-1免疫反应性的鉴定与表征
Endocrinology. 2009 Jan;150(1):232-8. doi: 10.1210/en.2008-0747. Epub 2008 Sep 25.
10
Regulation of gastric nesfatin-1/NUCB2.胃促生长素-1/核结合蛋白2的调节
Curr Pharm Des. 2013;19(39):6981-5. doi: 10.2174/138161281939131127143306.

引用本文的文献

1
Gastric GDF15 levels are regulated by age, sex, and nutritional status in rodents and humans.胃泌素释放肽 15（Gastric GDF15）在啮齿动物和人类中的水平受年龄、性别和营养状况的调节。
J Endocrinol Invest. 2024 May;47(5):1139-1154. doi: 10.1007/s40618-023-02232-y. Epub 2023 Nov 13.
2
Signaling pathways in obesity: mechanisms and therapeutic interventions.肥胖症中的信号通路：机制与治疗干预。
Signal Transduct Target Ther. 2022 Aug 28;7(1):298. doi: 10.1038/s41392-022-01149-x.
3
mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4 T Cell Apoptosis Mediated by ROS in Septic Immunosuppression.

本文引用的文献

1
Current Understanding of the Hypothalamic Ghrelin Pathways Inducing Appetite and Adiposity.目前对下丘脑Ghrelin 通路引起食欲和肥胖的理解。
Trends Neurosci. 2017 Mar;40(3):167-180. doi: 10.1016/j.tins.2016.12.003. Epub 2017 Jan 17.
2
The Tissue Distribution of Nesfatin-1/NUCB2 in Mouse.Nesfatin-1/NUCB2在小鼠体内的组织分布
Dev Reprod. 2014 Dec;18(4):301-9. doi: 10.12717/devrep.2014.18.4.301.
3
The gastric CB1 receptor modulates ghrelin production through the mTOR pathway to regulate food intake.胃 CB1 受体通过 mTOR 途径调节生长激素释放肽的产生，从而调节食物摄入。
mTOR 调节 ROS 介导的内质网应激诱导的 CD4 T 细胞凋亡在脓毒症免疫抑制中的作用。
Mediators Inflamm. 2022 Jul 23;2022:6077570. doi: 10.1155/2022/6077570. eCollection 2022.
4
The Multifaceted Nature of Nucleobindin-2 in Carcinogenesis.核结合蛋白-2在肿瘤发生中的多面性
Int J Mol Sci. 2021 May 26;22(11):5687. doi: 10.3390/ijms22115687.
5
mTOR Pathway is Involved in Energy Homeostasis Regulation as a Part of the Gut-Brain Axis.mTOR 通路作为肠脑轴的一部分参与能量稳态调节。
Int J Mol Sci. 2020 Aug 10;21(16):5715. doi: 10.3390/ijms21165715.
6
Current Understanding of the Role of Nesfatin-1.对Nesfatin-1作用的当前认识。
J Endocr Soc. 2018 Sep 10;2(10):1188-1206. doi: 10.1210/js.2018-00246. eCollection 2018 Oct 1.
PLoS One. 2013 Nov 26;8(11):e80339. doi: 10.1371/journal.pone.0080339. eCollection 2013.
4
mTOR-dependent modulation of gastric nesfatin-1/NUCB2.胃饥饿素-1/核结合蛋白2的mTOR依赖性调节
Cell Physiol Biochem. 2012;29(3-4):493-500. doi: 10.1159/000338503. Epub 2012 Apr 3.
5
Cellular distribution, regulated expression, and functional role of the anorexigenic peptide, NUCB2/nesfatin-1, in the testis.在睾丸中，摄食抑制肽 NUCB2/nesfatin-1 的细胞分布、调节表达和功能作用。
Endocrinology. 2012 Apr;153(4):1959-71. doi: 10.1210/en.2011-2032. Epub 2012 Feb 14.
6
Gastric mammalian target of rapamycin signaling regulates ghrelin production and food intake.雷帕霉素的胃哺乳动物靶点信号传导调节胃饥饿素的产生和食物摄入。
Endocrinology. 2009 Aug;150(8):3637-44. doi: 10.1210/en.2009-0372. Epub 2009 Apr 30.
7
Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism.外周给予nesfatin-1可减少小鼠的食物摄入量：不依赖瘦素的机制。
Endocrinology. 2009 Feb;150(2):662-71. doi: 10.1210/en.2008-0598. Epub 2008 Oct 16.
8
Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa.大鼠胃泌酸黏膜内分泌细胞类型中nesfatin-1免疫反应性的鉴定与表征
Endocrinology. 2009 Jan;150(1):232-8. doi: 10.1210/en.2008-0747. Epub 2008 Sep 25.
9
Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain.内脂素-1：在大鼠脑中的分布及其与G蛋白偶联受体的相互作用
Endocrinology. 2007 Oct;148(10):5088-94. doi: 10.1210/en.2007-0701. Epub 2007 Jul 12.
10
Sensory stimuli directly acting at the central nervous system regulate gastric ghrelin secretion. an ex vivo organ culture study.直接作用于中枢神经系统的感觉刺激调节胃饥饿素分泌。一项体外器官培养研究。
Endocrinology. 2007 Aug;148(8):3998-4006. doi: 10.1210/en.2007-0226. Epub 2007 May 10.