Ohara Nobuyoshi, Haraguchi Naotsugu, Koseki Jun, Nishizawa Yujiro, Kawai Kenji, Takahashi Hidekazu, Nishimura Junichi, Hata Taishi, Mizushima Tsunekazu, Yamamoto Hirofumi, Ishii Hideshi, Doki Yuichiro, Mori Masaki
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
Department of Cancer Profiling Discovery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
Oncol Lett. 2017 Oct;14(4):4483-4490. doi: 10.3892/ol.2017.6817. Epub 2017 Aug 24.
The Golgi-associated PDZ- and coiled-coil motif-containing (GOPC) protein controls the intracellular trafficking of numerous integral membrane proteins. Knockdown of GOPC increases activation of the mitogen-activated protein kinase-extracellular signal-regulated kinase 1/2 pathway and cancer cell progression in colorectal cancer. The present study aimed to clarify the correlation between GOPC expression and prognosis in colorectal cancer. Total RNA was extracted from 153 clinical colorectal cancer specimens and GOPC expression was evaluated using reverse transcription-quantitative polymerase chain reaction. The correlation between GOPC expression and clinicopathological factors was analyzed, along with the association of GOPC expression with overall survival (OS) and with recurrence-free survival (RFS). Lower expression of GOPC was significantly associated with a high frequency of venous invasion (P=0.001) and to poorer OS and RFS based on Kaplan-Meier analysis. In addition, multivariate analyses using a Cox proportional hazards model identified lower expression of GOPC to be an independent prognostic factor for colorectal cancer (hazard ratio=2.800; 95% confidence interval; 1.121-7.648; P=0.027). Lower expression of GOPC revealed a high frequency of venous invasion and associated with poorer prognosis for patients with colorectal cancer.
高尔基体相关的含PDZ和卷曲螺旋基序（GOPC）的蛋白质控制着众多整合膜蛋白的细胞内运输。敲低GOPC会增加丝裂原活化蛋白激酶-细胞外信号调节激酶1/2通路的激活以及结直肠癌中的癌细胞进展。本研究旨在阐明GOPC表达与结直肠癌预后之间的相关性。从153例临床结直肠癌标本中提取总RNA，并使用逆转录-定量聚合酶链反应评估GOPC表达。分析了GOPC表达与临床病理因素之间的相关性，以及GOPC表达与总生存期（OS）和无复发生存期（RFS）的关联。基于Kaplan-Meier分析，GOPC的低表达与高频率的静脉侵犯显著相关（P = 0.001），并且与较差的OS和RFS相关。此外，使用Cox比例风险模型进行的多变量分析确定GOPC的低表达是结直肠癌的独立预后因素（风险比= 2.800；95％置信区间；1.121 - 7.648；P = 0.027）。GOPC的低表达显示出高频率的静脉侵犯，并与结直肠癌患者较差的预后相关。
