Nikanfar Raha, Dabbaghi Rozhin, Rajabi Ali, Hashemzadeh Shahriar, Baradaran Behzad, Teimourian Shahram, Safaralizadeh Reza
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of General and Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Biomed Res. 2023 Jul 20;12:186. doi: 10.4103/abr.abr_260_22. eCollection 2023.
Long non-coding RNAs (lncRNAs) have emerged as crucial regulators in various biological processes, including cancer development and progression. This study aimed to investigate the expression differences of the BRAF-activated non-coding RNA (BANCR) gene in GC tissues compared to adjacent normal tissues. The potential diagnostic significance of BANCR in GC was explored, with the aim of improving diagnostic and therapeutic approaches for this global health burden.
Tissue samples from 100 gastric cancer (GC) patients were collected, and BANCR expression was analyzed using quantitative real-time PCR. Correlations between BANCR expression and clinicopathological features were assessed, and its biomarker potential was evaluated.
In individuals diagnosed with GC, the expression of BANCR was notably elevated in tumor tissues compared to adjacent normal tissues ( < 0.0001). However, the analysis of gene expression data did not demonstrate any statistically significant correlation between elevated BANCR expression and clinicopathological features. According to the ROC analysis, BANCR demonstrated an AUC of 0.6733 ( < 0.0001), with a sensitivity of 73% and a specificity of 45%. However, further evaluation is required to determine its potential as a biomarker (CI 95% = 0.5992 to 0.7473).
The observed upregulation of BANCR in GC tissues implies its potential involvement as an oncogenic lncRNA in GC patients. Furthermore, BANCR may serve as a promising biomarker for identification and treatment of GC.
长链非编码RNA（lncRNAs）已成为包括癌症发生和发展在内的各种生物学过程中的关键调节因子。本研究旨在调查BRAF激活的非编码RNA（BANCR）基因在胃癌组织与相邻正常组织中的表达差异。探讨了BANCR在胃癌中的潜在诊断意义，旨在改善针对这一全球健康负担的诊断和治疗方法。
收集100例胃癌（GC）患者的组织样本，采用定量实时PCR分析BANCR表达。评估BANCR表达与临床病理特征之间的相关性，并评估其作为生物标志物的潜力。
在被诊断为GC的个体中，与相邻正常组织相比，肿瘤组织中BANCR的表达显著升高（<0.0001）。然而，基因表达数据分析未显示BANCR表达升高与临床病理特征之间存在任何统计学上的显著相关性。根据ROC分析，BANCR的AUC为0.6733（<0.0001），敏感性为73%，特异性为45%。然而，需要进一步评估以确定其作为生物标志物的潜力（95%CI=0.5992至0.7473）。
在GC组织中观察到的BANCR上调意味着其可能作为一种致癌lncRNA参与GC患者的发病过程。此外，BANCR可能是一种有前景的用于GC识别和治疗的生物标志物。
