Ghatei Najmeh, Nabavi Ariane Sadr, Toosi Mohammad Hossein Bahreyni, Azimian Hosein, Homayoun Mansour, Targhi Reza Ghasemnezhad, Haghir Hossein
Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2017 Sep;20(9):1037-1043. doi: 10.22038/IJBMS.2017.9273.
The increasing rate of over using cell phones has been considerable in youths and pregnant women. We examined the effect of mobile phones radiation on genes expression variation on cerebellum of BALB/c mice before and after of the birth.
In this study, a mobile phone jammer, which is an instrument to prevent receiving signals between cellular phones and base transceiver stations (two frequencies 900 and 1800 MHz) for exposure was used and twelve pregnant mice (BALB/c) divided into two groups (n=6), first group irradiated in pregnancy period (19th day), the second group did not irradiate in pregnancy period. After childbirth, offspring were classified into four groups (n=4): Group1: control, Group 2: B1 (Irradiated after birth), Group 3: B2 (Irradiated in pregnancy period and after birth), Group 4: B3 (Irradiated in pregnancy period). When maturity was completed (8-10 weeks old), mice were dissected and cerebellum was isolated. The expression level of , bcl-2, p21 and genes examined by real-time reverse transcription polymerase chain reaction (Real-Time RT- PCR).
The data showed that mobile phone radio waves were ineffective on the expression level of bcl-2 and p53 genes) >0.05(. Also gene expression level of decreased and gene expression level of increased comparing to the control group (<0.05).
From the obtained data it could be concluded that the mobile phone radiations did not induce apoptosis in cells of the cerebellum and the injured cells can be repaired by cell cycle arrest.
青少年和孕妇过度使用手机的比例一直在显著上升。我们研究了手机辐射对BALB/c小鼠出生前后小脑基因表达变化的影响。
在本研究中，使用了一种手机信号干扰器，这是一种用于暴露实验的防止手机与基站之间接收信号的仪器（两个频率900和1800兆赫），将12只怀孕的BALB/c小鼠分为两组（n = 6），第一组在孕期（第19天）接受辐射，第二组在孕期不接受辐射。分娩后，后代被分为四组（n = 4）：第1组：对照组，第2组：B1（出生后接受辐射），第3组：B2（孕期和出生后均接受辐射），第4组：B3（孕期接受辐射）。当小鼠成熟（8 - 10周龄）时，进行解剖并分离出小脑。通过实时逆转录聚合酶链反应（实时RT - PCR）检测 、bcl - 2、p21和 基因的表达水平。
数据显示手机无线电波对bcl - 2和p53基因的表达水平无影响（>0.05）。与对照组相比， 基因表达水平降低， 基因表达水平升高（<0.05）。
从获得的数据可以得出结论，手机辐射不会诱导小脑细胞凋亡，受损细胞可通过细胞周期停滞进行修复。
