Baştu Ercan, Parlatan Uğur, Başar Günay, Yumru Harika, Bavili Nima, Sağ Fatih, Bulgurcuoğlu Sibel, Buyru Faruk
İstanbul University İstanbul Faculty of Medicine, Department of Obstetrics and Gynecology, İstanbul, Turkey.
İstanbul Technical University Faculty of Science and Letter, Department of Physics Engineering, İstanbul, Turkey.
Turk J Obstet Gynecol. 2017 Sep;14(3):145-150. doi: 10.4274/tjod.92604. Epub 2017 Sep 30.
To predict the reproductive potential of embryos via Raman spectroscopy evaluation of the spent culture media as well as with a conventional morphologic evaluation.
Women of reproductive age (n=31) who were treated for unexplained infertility and scheduled for single embryo transfer were invited to participate in this prospective study. After the embryos were removed from the culture, the spent culture media were stored at -80 °C after snap-freezing in liquid nitrogen.
Fifteen patients were clinically pregnant, and 16 patients were clinically non-pregnant. Clinical pregnancy was predicted using Raman spectroscopy in 93% (14/15) of clinically pregnant patients, and in 62.5% (10 out of 16) of clinically non-pregnant patients. The sensitivity of the Raman spectroscopic analysis was 93% and the specificity was 62.5%.
Metabolomic evaluation of spent embryo culture media is an emerging technique with promising objective results. However, there is clearly room for improvement.
通过对废弃培养基进行拉曼光谱评估以及传统形态学评估来预测胚胎的生殖潜力。
邀请因不明原因不孕症接受治疗并计划进行单胚胎移植的育龄妇女（n = 31）参与这项前瞻性研究。胚胎从培养中取出后，废弃培养基在液氮中速冻后储存在-80°C。
15例患者临床妊娠，16例患者临床未妊娠。使用拉曼光谱预测临床妊娠的准确率在临床妊娠患者中为93%（14/15），在临床未妊娠患者中为62.5%（16例中的10例）。拉曼光谱分析的敏感性为93%，特异性为62.5%。
对废弃胚胎培养基进行代谢组学评估是一项新兴技术，具有可观的客观结果。然而，显然仍有改进空间。
