Diekmann Robin, Till Katharina, Müller Marcel, Simonis Matthias, Schüttpelz Mark, Huser Thomas
Department of Physics, Bielefeld University, Bielefeld, Germany.
Micron Oxford, Department of Biochemistry, University of Oxford, Oxford, UK.
Sci Rep. 2017 Oct 31;7(1):14425. doi: 10.1038/s41598-017-14762-6.
Many commercial as well as custom-built fluorescence microscopes use scientific-grade cameras that represent a substantial share of the instrument's cost. This holds particularly true for super-resolution localization microscopy where high demands are placed especially on the detector with respect to sensitivity, noise, and also image acquisition speed. Here, we present and carefully characterize an industry-grade CMOS camera as a cost-efficient alternative to commonly used scientific cameras. Direct experimental comparison of these two detector types shows widely similar performance for imaging by single molecule localization microscopy (SMLM). Furthermore, high image acquisition speeds are demonstrated for the CMOS detector by ultra-fast SMLM imaging.
许多商用以及定制的荧光显微镜都使用科学级相机，这些相机在仪器成本中占相当大的比例。对于超分辨率定位显微镜来说尤其如此，在超分辨率定位显微镜中，对探测器在灵敏度、噪声以及图像采集速度方面有着特别高的要求。在此，我们展示并详细表征了一款工业级互补金属氧化物半导体（CMOS）相机，它可作为常用科学相机的一种经济高效的替代方案。对这两种探测器类型的直接实验比较表明，在单分子定位显微镜（SMLM）成像方面，它们的性能广泛相似。此外，通过超快速SMLM成像证明了CMOS探测器具有高图像采集速度。
