Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, 430074, China.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
Cytometry A. 2017 Dec;91(12):1175-1183. doi: 10.1002/cyto.a.23282. Epub 2017 Nov 22.
Scientific Complementary Metal Oxide Semiconductor (sCMOS) cameras were introduced into the market in 2009 and are now becoming a major type of commercial cameras for low-light imaging. sCMOS cameras provide simultaneously low read noise, high readout speed, and large pixel array; however, the relatively low quantum efficiency (QE) of sCMOS cameras has been a major limitation for its application in single molecule imaging, especially super-resolution localization microscopy which requires high detection sensitivity. Here we report the imaging performance of a newly released back-illuminated sCMOS camera (called Dhyana 95 from Tucsen) which is claimed to be the world's first 95% QE sCMOS camera. The imaging performance evaluation is based on a new methodology which is designed to provide paired images from two tested cameras under almost identical experimental conditions. We verified that this new 95% QE sCMOS camera is able to provide superior imaging performance over a representative front-illuminated sCMOS camera (Hamamatsu Flash 4.0 V2) and a popular back-illuminated EMCCD camera (Andor iXon 897 Ultra) in a wide signal range. We hope this study will inspire more studies on using sCMOS cameras in super-resolution localization microscopy, or even single molecule imaging. © 2017 International Society for Advancement of Cytometry.
2009 年，科研型互补金属氧化物半导体（sCMOS）相机进入市场，现已成为用于弱光成像的主要商用相机类型。sCMOS 相机具有低读出噪声、高读出速度和大像素阵列等特点；然而，sCMOS 相机相对较低的量子效率（QE）一直是其在单分子成像应用中的主要限制因素，特别是对于需要高检测灵敏度的超分辨率定位显微镜而言。本文报道了一款新推出的背照式 sCMOS 相机（Tucsen 公司的 Dhyana 95）的成像性能，据称这是世界上首款量子效率达到 95%的 sCMOS 相机。该成像性能评估基于一种新方法，旨在在几乎相同的实验条件下，从两台经过测试的相机中获取配对图像。我们验证了这款新的 95%量子效率 sCMOS 相机在广泛的信号范围内，相较于一款具有代表性的前照式 sCMOS 相机（Hamamatsu Flash 4.0 V2）和一款流行的背照式 EMCCD 相机（Andor iXon 897 Ultra），具有更优异的成像性能。我们希望这项研究能够激发更多关于在超分辨率定位显微镜中，甚至是在单分子成像中使用 sCMOS 相机的研究。© 2017 国际细胞分析学会。
