基于定位的超分辨率显微镜与sCMOS相机第二部分：比较sCMOS相机与EMCCD相机的实验方法

Localization-based super-resolution microscopy with an sCMOS camera part II: experimental methodology for comparing sCMOS with EMCCD cameras.

作者信息

Long Fan, Zeng Shaoqun, Huang Zhen-Li

机构信息

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.

出版信息

Opt Express. 2012 Jul 30;20(16):17741-59. doi: 10.1364/OE.20.017741.

DOI:10.1364/OE.20.017741

PMID:23038326

Abstract

Nowadays, there is a hot debate among industry and academic researchers that whether the newly developed scientific-grade Complementary Metal Oxide Semiconductor (sCMOS) cameras could become the image sensors of choice in localization-based super-resolution microscopy. To help researchers find answers to this question, here we reported an experimental methodology for quantitatively comparing the performance of low-light cameras in single molecule detection (characterized via image SNR) and localization (via localization accuracy). We found that a newly launched sCMOS camera can present superior imaging performance than a popular Electron Multiplying Charge Coupled Device (EMCCD) camera in a signal range (15-12000 photon/pixel) more than enough for typical localization-based super-resolution microscopy.

摘要

如今，行业和学术研究人员之间正在热烈争论新开发的科学级互补金属氧化物半导体（sCMOS）相机是否会成为基于定位的超分辨率显微镜中首选的图像传感器。为了帮助研究人员找到这个问题的答案，我们在此报告了一种实验方法，用于定量比较低光相机在单分子检测（通过图像信噪比表征）和定位（通过定位精度）方面的性能。我们发现，在一个对于典型的基于定位的超分辨率显微镜来说绰绰有余的信号范围（15 - 12000光子/像素）内，一款新推出的sCMOS相机能够呈现出比一款流行的电子倍增电荷耦合器件（EMCCD）相机更优异的成像性能。