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氧化锌纳米棒与共生真菌DSM 11827的协同联合对西兰花灰霉病菌的影响

Impact of Synergistic Association of ZnO-Nanorods and Symbiotic Fungus DSM 11827 on var. botrytis (Broccoli).

作者信息

Singhal Uma, Khanuja Manika, Prasad Ram, Varma Ajit

机构信息

Amity Institute of Microbial Technology, Amity University, Noida, India.

Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia, New Delhi, India.

出版信息

Front Microbiol. 2017 Oct 17;8:1909. doi: 10.3389/fmicb.2017.01909. eCollection 2017.

Abstract

In the present work, novel nanotool called 'nano-embedded fungus' formed by impact of synergistic association of ZnO-nanorods and fungus DSM 11827, for growth of var. botrytis (Broccoli) is reported. ZnO-nanorods were synthesized by mechanical assisted thermal decomposition process and characterized by scanning electron microscopy (SEM) for morphology, X-ray diffraction for structural studies and UV-vis absorption spectroscopy for band gap determination. Nanoembedded fungus is prepared by optimizing ZnO-nanorods concentration (500 ppm) which resulted in the increased biomass of , as confirmed by dry weight method, spore count, spread plate and microscopy techniques viz. SEM and confocal microscopy. Enhancement in var. botrytis is reported on treatment with nanoembedded fungus. According to the authors, this is the first holistic study focusing on the impact of ZnO-nanorods in the enhancement of fungal symbiont for enhanced biomass productivity of plant.

摘要

在本研究中,报道了一种名为“纳米嵌入真菌”的新型纳米工具,它是由氧化锌纳米棒与真菌DSM 11827协同结合形成的,用于促进葡萄孢菌(西兰花)的生长。通过机械辅助热分解法合成了氧化锌纳米棒,并通过扫描电子显微镜(SEM)对其形态进行表征,通过X射线衍射进行结构研究,通过紫外可见吸收光谱确定带隙。通过优化氧化锌纳米棒浓度(500 ppm)制备了纳米嵌入真菌,通过干重法、孢子计数、平板涂布和显微镜技术(即SEM和共聚焦显微镜)证实,这导致了葡萄孢菌生物量的增加。据报道,用纳米嵌入真菌处理可促进葡萄孢菌生长。作者称,这是第一项全面研究氧化锌纳米棒对增强真菌共生体以提高植物生物量生产力影响的研究。

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