Ryu Yong Ah, Jin Meiying, Kang Nakheon
Department of Plastic and Reconstructive Surgery, Chungnam National University School of Medicine, Daejeon, Korea.
Arch Craniofac Surg. 2017 Sep;18(3):155-161. doi: 10.7181/acfs.2017.18.3.155. Epub 2017 Sep 26.
Because of the relatively similar size of organs to human and the physiological and structural similarities, the use of porcine as xenograft donors is progressing very actively. In this study, we analyzed the characteristics of porcine ear cartilage and evaluated its suitability as graft material in reconstructive and cosmetic surgery.
The auricular cartilage was harvested from two pigs, and subjected to histological examination by immunohistochemical staining. To determine the collagen content, samples were treated with collagenase and weight changes were measured. After sterilization by irradiation, the samples were grafted into rats and stained with Hematoxylin and Eosin and Masson Trichrome to observe inflammation and xenograft rejection.
In IHC staining, extracellular matrices were mainly stained with type II collagen (20.69%), keratin sulfate (10.20%), chondroitin sulfate (2.62%), and hyaluronic acid (0.84%). After collagenase treatment, the weight decreased by 68.3%, indicating that about 70% of the porcine ear cartilage was composed of collagen. Upon xenograft of the sterilized cartilages in rats, inflammatory cells were observed for up to 2 months. However, they gradually decreased, and inflammation and reject-response were rarely observed at 5 months.
The porcine ear cartilage was covered with perichondrium and cellular constituents were found to be composed of chondrocytes and chondroblasts. In addition, the extracellular matrices were mainly composed of collagen. Upon xenograft of irradiated cartilage into rats, there was no specific inflammatory reaction around the transplanted cartilage. These findings suggest that porcine ear cartilage could be a useful alternative implant material for human cosmetic surgery.
由于猪的器官在大小上与人类相对相似，且在生理和结构上也有相似之处，因此将猪作为异种移植供体的应用正在积极推进。在本研究中，我们分析了猪耳软骨的特性，并评估了其作为重建和美容手术中移植材料的适用性。
从两头猪身上获取耳廓软骨，通过免疫组织化学染色进行组织学检查。为了测定胶原蛋白含量，将样本用胶原酶处理并测量重量变化。经辐照灭菌后，将样本移植到大鼠体内，并用苏木精-伊红和马松三色染色法观察炎症和异种移植排斥反应。
在免疫组织化学染色中，细胞外基质主要被II型胶原蛋白（20.69%）、硫酸角质素（10.20%）、硫酸软骨素（2.62%）和透明质酸（0.84%）染色。经胶原酶处理后，重量下降了68.3%，表明约70%的猪耳软骨由胶原蛋白组成。将灭菌后的软骨异种移植到大鼠体内后，在长达2个月的时间内观察到炎症细胞。然而，它们逐渐减少，在5个月时很少观察到炎症和排斥反应。
猪耳软骨被软骨膜覆盖，细胞成分由软骨细胞和成软骨细胞组成。此外，细胞外基质主要由胶原蛋白组成。将辐照后的软骨异种移植到大鼠体内后，移植软骨周围没有特异性炎症反应。这些发现表明，猪耳软骨可能是人类美容手术中一种有用的替代植入材料。
