Wang Chunrong, Zhang Mengru, Cheng Fanglin, Geng Qi
a School of Chemical and Environmental Engineering, China University of Mining and Technology , Beijing , P.R. China.
Biosci Biotechnol Biochem. 2015;79(1):164-70. doi: 10.1080/09168451.2014.952615. Epub 2014 Sep 1.
A novel quinoline-degrading strain, named K4, was isolated from activated sludge of a coking wastewater treatment plant and identified as Brevundimonas sp. on the basis of its 16s rDNA gene sequence analysis. Its optimum temperature and pH for quinoline degradation were 30 °C and pH 9.0, respectively, and during the biodegradation process, at 100 mg/L initial quinoline concentration, an inoculation amount of 8% (OD600 of 0.23) was the optimal strain concentration. In addition, the kinetics of free K4 strains for quinoline degradation showed that it followed a zero-order equation. Furthermore, compared with free K4 strains, immobilized K4 strains' potential for quinoline degradation was investigated by adding both of them into SBR reactors for actual coking wastewater treatment on operation over 15 days. The results showed that bioaugmentation by both free and immobilized K4 strains enhanced quinoline removal efficiency, and especially, the latter could reach its stable removal after a shorter accommodation period, with 94.8% of mean quinoline removal efficiency.
从某焦化废水处理厂的活性污泥中分离出一株新型喹啉降解菌株，命名为K4，并通过16s rDNA基因序列分析将其鉴定为短波单胞菌属。其喹啉降解的最适温度和pH分别为30℃和pH 9.0，在生物降解过程中，初始喹啉浓度为100mg/L时，接种量为8%（OD600为0.23）是最佳菌株浓度。此外，游离K4菌株对喹啉降解的动力学表明其遵循零级方程。此外，将游离K4菌株和固定化K4菌株分别加入SBR反应器中处理实际焦化废水15天，研究了固定化K4菌株与游离K4菌株相比的喹啉降解潜力。结果表明，游离和固定化K4菌株的生物强化均提高了喹啉去除效率，尤其是后者在较短的适应期后即可达到稳定去除，平均喹啉去除效率为94.8%。
