College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8475-8484. doi: 10.1007/s00253-017-8600-2. Epub 2017 Oct 31.
Low pathogenic H9N2 subtype avian influenza virus (AIV) can lead to moderate respiratory symptoms and low egg production rates in poultry. Due to its immunologic suppression, other various infectious pathogens give rise to the co-infection of hosts, causing heavy economic losses in the commercial poultry industry in both China and worldwide. Therefore, it is time to explore a novel, safe, and effective vaccine. We have already made use of the surface of Lactobacillus plantarum to display AIV HA2 (NC8-pSIP409-pgsA'-HA2), which demonstrated that it has a good immunogenicity. In this study, by evaluating the immune protection effect of NC8-pSIP409-pgsA'-HA2 on chickens, we found that the hemagglutination inhibition (HI) antibodies, specificity IgG antibody in chickens, the sIgA titer in broncho alveolar lavage fluids (BALF), and the T cell response were increased notably after oral vaccination with NC8-pSIP409-pgsA'-HA2. In addition, weight loss, lung titers, and lung pathologies were improved when chickens were orally vaccinated with NC8-pSIP409-pgsA'-HA2 after challenge with H9N2 AIV. This strategy lays the foundation for the development of recombinant L. plantarum oral vaccines in the prevention of AIV.
低致病性 H9N2 亚型禽流感病毒（AIV）可导致家禽出现中度呼吸道症状和产蛋率降低。由于其免疫抑制作用，其他各种传染性病原体导致宿主的合并感染，给中国和全球的商业家禽业造成了巨大的经济损失。因此，现在是时候探索一种新型、安全且有效的疫苗了。我们已经利用植物乳杆菌的表面展示了 AIV HA2（NC8-pSIP409-pgsA'-HA2），结果表明它具有良好的免疫原性。在这项研究中，我们通过评估 NC8-pSIP409-pgsA'-HA2 对鸡的免疫保护作用，发现经口服接种 NC8-pSIP409-pgsA'-HA2 后，鸡的血凝抑制（HI）抗体、特异性 IgG 抗体、支气管肺泡灌洗液（BALF）中的 sIgA 滴度和 T 细胞反应明显增加。此外，当鸡在受到 H9N2 AIV 攻击后经口服接种 NC8-pSIP409-pgsA'-HA2 时，体重减轻、肺部滴度和肺部病变得到改善。这项策略为开发用于预防 AIV 的重组植物乳杆菌口服疫苗奠定了基础。
