Wang Lin-Jian, Wan Bo, Guo Zhenhua, Qiao Songlin, Li Rui, Xie Sha, Chen Xin-Xin, Zhang Gaiping
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China.
Virus Genes. 2018 Feb;54(1):86-97. doi: 10.1007/s11262-017-1516-1. Epub 2017 Nov 1.
Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macrophages from Henan province and the full-length genome sequence was determined. Phylogenetic analysis indicated that HNhx strain was classified into the NADC30-like PRRSV subgroup, in which all the strains had the unique discontinuous 131-amino acid deletion relative to that of the nonstructural protein 2 (Nsp2) of the VR2332 strain. Genetically, HNhx shared 92.9% nucleotide similarity to NADC30. Furthermore, HNhx strain contained extensive amino acid mutations in GP5. In particular, the S32H, N33D, D34N, and S36G variations resulted in that HNhx lost all the putative N-linked glycosylation sites at amino acid positions 30, 32, 33, 34, and 35. Recombination analysis revealed that HNhx was the result of recombination between the NADC30 strain and the highly pathogenic PRRSV vaccine strain circulating in China in Nsp4 (nt 5261) to Nsp9 (nt 7911). The novel genome data of HNhx will be helpful for understanding the evolution and epidemiology of PRRSV in China.
最近，与2008年在美国分离出的NADC30毒株基因相似的类NADC30猪繁殖与呼吸综合征病毒（PRRSV）在中国变得流行起来。在此，从河南省猪肺泡巨噬细胞中成功分离出一株名为HNhx的新型变异PRRSV毒株，并测定了其全长基因组序列。系统发育分析表明，HNhx毒株被归类为类NADC30 PRRSV亚组，该亚组中的所有毒株相对于VR2332毒株的非结构蛋白2（Nsp2）都有独特的131个氨基酸的不连续缺失。在基因上，HNhx与NADC30的核苷酸相似性为92.9%。此外，HNhx毒株在GP5中含有大量氨基酸突变。特别是，S32H、N33D、D34N和S36G变异导致HNhx在氨基酸位置30、32、33、34和35处失去了所有推定的N-糖基化位点。重组分析显示，HNhx是NADC30毒株与在中国流行的高致病性PRRSV疫苗株在Nsp4（nt 5261）至Nsp9（nt 7911）之间重组的结果。HNhx的新基因组数据将有助于了解PRRSV在中国的进化和流行病学情况。
