1 Plant Pathology, Institute of Integrative Biology, ETH Zurich, 8092 Zurich, Switzerland; and.
2 Department of Plant and Microbial Biology, University of Zurich, Zurich 8008, Switzerland.
Mol Plant Microbe Interact. 2018 Apr;31(4):420-431. doi: 10.1094/MPMI-10-17-0245-R. Epub 2018 Feb 13.
The fungus Zymoseptoria tritici causes septoria tritici blotch (STB) on wheat, an important disease globally and the most damaging wheat disease in Europe. Despite the global significance of STB, the molecular basis of wheat defense against Z. tritici is poorly understood. Here, we use a comparative transcriptomic study to investigate how wheat responds to infection by four distinct strains of Z. tritici. We examined the response of wheat across the entire infection cycle, identifying both shared responses to the four strains and strain-specific responses. We found that the early asymptomatic phase is characterized by strong upregulation of genes encoding receptor-like kinases and pathogenesis-related proteins, indicating the onset of a defense response. We also identified genes that were differentially expressed among the four fungal strains, including genes related to defense. Genes involved in senescence were induced during both the asymptomatic phase and at late stages of infection, suggesting manipulation of senescence processes by both the plant and the pathogen. Our findings illustrate the need, when identifying important genes affecting disease resistance in plants, to include multiple pathogen strains.
小麦壳针孢引起的叶枯病（STB）是一种重要的全球性病害，也是欧洲小麦最具破坏性的病害。尽管 STB 具有全球性意义，但小麦对 Zymoseptoria tritici 的防御的分子基础仍知之甚少。在这里，我们使用比较转录组学研究来研究小麦如何对四种不同的 Zymoseptoria tritici 菌株的感染作出反应。我们在整个感染周期中检查了小麦的反应，确定了对四种菌株的共同反应和菌株特异性反应。我们发现，早期无症状阶段的特征是编码受体样激酶和病程相关蛋白的基因强烈上调，表明防御反应的开始。我们还鉴定了在四种真菌菌株中差异表达的基因，包括与防御相关的基因。在无症状阶段和感染后期，与衰老相关的基因被诱导，这表明植物和病原体都对衰老过程进行了操纵。我们的研究结果表明，在鉴定影响植物抗病性的重要基因时，有必要包括多个病原菌菌株。
