Pauvolid-Corrêa Alex, Campos Zilca, Soares Raquel, Nogueira Rita Maria Ribeiro, Komar Nicholas
Arbovirus Diseases Branch, Centers for Disease Control and Prevention (CDC), Fort Collins, CO, United States of America.
Embrapa Pantanal, Ministério da Agricultura Pecuária e Abastecimento, Corumbá, MS, Brasil.
PLoS Negl Trop Dis. 2017 Nov 1;11(11):e0006014. doi: 10.1371/journal.pntd.0006014. eCollection 2017 Nov.
The Pantanal is a hotspot for arbovirus studies in South America. Various medically important flaviviruses and alphaviruses have been reported in domestic and wild animals in the region. To expand the knowledge of local arbovirus circulation, a serosurvey for 14 Brazilian orthobunyaviruses was conducted with equines, sheep and free-ranging caimans. Sera were tested for specific viral antibodies using plaque-reduction neutralization test (PRNT). Monotypic reactions were detected for Maguari, Xingu, Apeu, Guaroa, Murutucu, Oriboca, Oropouche and Nepuyo viruses. Despite the low titers for most of the orthobunyaviruses tested, the detection of monotypic reactions for eight orthobunyaviruses suggests the Pantanal as a region of great orthobunyavirus diversity. The present data, in conjunction with previous studies that detected a high diversity of other arboviruses, ratify the Pantanal as an important natural reservoir for sylvatic and medically important arboviruses in Brazil.
潘塔纳尔湿地是南美洲虫媒病毒研究的热点地区。该地区的家畜和野生动物中已报告了多种具有重要医学意义的黄病毒和甲病毒。为了扩大对当地虫媒病毒传播情况的了解，对马、绵羊和野生凯门鳄进行了针对14种巴西正布尼亚病毒的血清学调查。使用蚀斑减少中和试验（PRNT）检测血清中的特异性病毒抗体。检测到马瓜里病毒、欣古病毒、阿佩乌病毒、瓜罗阿病毒、穆鲁图库病毒、奥里博卡病毒、奥罗普切病毒和内普约病毒的单型反应。尽管大多数检测的正布尼亚病毒滴度较低，但检测到8种正布尼亚病毒的单型反应表明潘塔纳尔湿地是正布尼亚病毒多样性极高的地区。目前的数据与之前检测到其他多种虫媒病毒高度多样性的研究相结合，证实潘塔纳尔湿地是巴西野生和具有重要医学意义的虫媒病毒的重要天然储存库。
