Pauvolid-Corrêa Alex, Solberg Owen, Couto-Lima Dinair, Kenney Joan, Serra-Freire Nicolau, Brault Aaron, Nogueira Rita, Langevin Stanley, Komar Nicholas
Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Ministério da Saúde, Rio de Janeiro, RJ, Brazil,
Arch Virol. 2015 Jan;160(1):21-7. doi: 10.1007/s00705-014-2219-8. Epub 2014 Sep 25.
We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
我们描述了一种新型黄病毒的分离情况，该病毒是从2010年在巴西中西部潘塔纳尔地区采集的一群被鉴定为克氏库蚊（库蚊属）的蚊子中分离出来的。该病毒在此以采集蚊子样本的牧场名称命名为努米林病毒（NHUV）。通过对匀浆后的蚊子以及相应的C6/36培养上清液进行实时逆转录聚合酶链反应（RT-PCR）检测到了黄病毒RNA。基于全基因组测序，该病毒分离株在基因上与塞内加尔新描述的巴尔克吉病毒（BJV）不同，但关系最为密切。系统发育分析表明，NHUV与蚊媒黄病毒归为一类，与BJV形成一个进化枝。这个进化枝在基因上可能介于鸟类传播的库蚊传播黄病毒和仅在昆虫中传播的黄病毒之间。
