Queiroz Adriele A, França Eduardo L, Hara Cristiane C P, Honorio Mariana S, Fagundes Danny L G, Calderon Iracema M P, Honorio-França Adenilda C
a Institute of Biological and Health Science, Federal University of Mato Grosso , Barra do Garças , Brazil.
b Botucatu Medical School, Graduate Program in Gynecology, Obstetrics and Mastology, São Paulo State University/UNESP , Botucatu , Brazil.
J Matern Fetal Neonatal Med. 2019 Apr;32(7):1098-1104. doi: 10.1080/14767058.2017.1400004. Epub 2017 Nov 20.
Changes in regulatory T cells (Treg) in peripheral blood are associated with a number of pathologies, including diabetes. However, the immunological responses of pregnant diabetic women remain scarcely known, and the effects of Treg cells in these patients have yet to be investigated. The present study characterized the expression of regulatory T cells in the maternal blood, cord blood and placenta of diabetic pregnant women. The women were divided according to glycemic status into a non-diabetic (ND; = 20) or type 2 diabetic (T2DM; = 20) group. Cell subsets were determined by flow cytometry. Compared to ND, T2DM blood cells exhibited a higher expression of CD25, Foxp3, CD4CD25, CD4Foxp3 and CD25Foxp3; and cord blood cells showed a lower expression of CD25, CD4Foxp3 and CD25Foxp3. In the placenta of T2DM, the villous layer of the proportion, CD3 and CD25 was lower than that of CD4Foxp3 and CD25Foxp3, and the extravillous placenta layer contained the lowest levels of CD4 and CD25 and highest proportions of CD4Foxp3. In maternal blood from T2DM, the frequency of CD3CD95 and CD3CD4 T cells expressing CD95 was lower. In cord blood from T2DM, the rate of CD3CD95 was lower. The placenta villous layer of T2DM showed a lower count of CD3CD95 and of CD3CD4 T cells expressing CD95, whereas the number of cells expressing CD3CD45RO decreased in both placental layers. The data obtained suggest that hyperglycemia changes the phenotypes of regulatory T cells and Fas expression in memory T cells.
外周血中调节性T细胞（Treg）的变化与包括糖尿病在内的多种病理状况相关。然而，妊娠糖尿病女性的免疫反应仍鲜为人知，Treg细胞在这些患者中的作用尚待研究。本研究对糖尿病孕妇的母血、脐血和胎盘中调节性T细胞的表达进行了表征。根据血糖状况将这些女性分为非糖尿病组（ND；n = 20）或2型糖尿病组（T2DM；n = 20）。通过流式细胞术确定细胞亚群。与ND组相比，T2DM组血细胞中CD25、Foxp3、CD4CD25、CD4Foxp3和CD25Foxp3的表达较高；脐血细胞中CD25、CD4Foxp3和CD25Foxp3的表达较低。在T2DM组的胎盘中，绒毛层中CD3和CD25的比例低于CD4Foxp3和CD25Foxp3，绒毛外胎盘层中CD4和CD25的水平最低，CD4Foxp3的比例最高。在T2DM组的母血中，表达CD95的CD3CD95和CD3CD4 T细胞频率较低。在T2DM组的脐血中，CD3CD95的比例较低。T2DM组胎盘绒毛层中表达CD95的CD3CD95和CD3CD4 T细胞数量较少，而在两个胎盘层中表达CD3CD45RO的细胞数量均减少。所获得的数据表明，高血糖会改变调节性T细胞的表型以及记忆T细胞中Fas的表达。
