越橘提取物对顺铂致大鼠耳毒性的保护作用。
The protective effects of whortleberry extract against cisplatin-induced ototoxicity in rats.
机构信息
University of Health Sciences, Samsun Health Practices and Research Center, Department of Otorhinolaryngology, Samsun, Turkey.
University of Health Sciences, Samsun Health Practices and Research Center, Department of Otorhinolaryngology, Samsun, Turkey.
出版信息
Braz J Otorhinolaryngol. 2019 Jan-Feb;85(1):55-62. doi: 10.1016/j.bjorl.2017.10.009. Epub 2017 Nov 10.
INTRODUCTION
Cisplatin is one of the main chemotherapeutic agents used for the treatment of many types of cancer. However, ototoxicity, one of the most serious side effects of cisplatin, restricts its usage.
OBJECTIVE
We aimed to investigate the protective effects of whortleberry extract against cisplatin-induced ototoxicity by evaluating hearing and histopathological cochlear damage and by measuring the biochemical parameters affected byoxidative stress.
METHODS
Forty-eight male rats were included in the study after performing Distortion Product Otoacoustic Emission test to confirm that their hearing levels were normal. The rats were randomly divided into six groups: the control group, the sham group, and, which received only whortleberry extract, only cisplatin, cisplatin+100mg whortleberry extract, cisplatin+200mg whortleberry extract, respectively. Audiologic investigation was performed by performing the Distortion Product Otoacoustic Emission test at the beginning and at the eighth day of the study. Cardiac blood samples were collected for biochemical analysis, and the rats were sacrificed to obtain cochlear histopathological specimens on the eighth day.
RESULTS
The results revealed that whortleberry protects hearing against cisplatin-induced ototoxicity independent of the dose. However, high doses of whortleberry extract are needed to prevent histopathological degeneration and oxidative stress.
CONCLUSION
The results obtained in this study show that whortleberry extract has a protective effect against cisplatin-induced ototoxicity.
简介
顺铂是用于治疗多种癌症的主要化疗药物之一。然而，顺铂的耳毒性是最严重的副作用之一，限制了其使用。
目的
我们旨在通过评估听力和组织病理学耳蜗损伤以及测量受氧化应激影响的生化参数，研究越桔提取物对顺铂诱导的耳毒性的保护作用。
方法
在进行畸变产物耳声发射测试以确认听力水平正常后，将 48 只雄性大鼠纳入研究。大鼠随机分为六组：对照组、假手术组、仅接受越桔提取物组、仅接受顺铂组、顺铂+100mg 越桔提取物组、顺铂+200mg 越桔提取物组。在研究开始和第 8 天进行畸变产物耳声发射测试进行听力调查。采集心脏血液样本进行生化分析，并在第 8 天处死大鼠以获得耳蜗组织病理学标本。
结果
结果表明，越桔提取物可独立于剂量保护听力免受顺铂诱导的耳毒性。然而，需要高剂量的越桔提取物来预防组织病理学退化和氧化应激。
结论
本研究结果表明，越桔提取物对顺铂诱导的耳毒性具有保护作用。
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