滑液中miR-140-3p和miR-140-5p的失调均与骨关节炎严重程度相关。
Dysregulation of both miR-140-3p and miR-140-5p in synovial fluid correlate with osteoarthritis severity.
作者信息
Yin C-M, Suen W-C-W, Lin S, Wu X-M, Li G, Pan X-H
机构信息
Shenzhen Bao'an People's Hospital, Department of Orthopaedics and Traumatology, Shenzhen Bao'an People's Hospital affiliated to Southern Medical University and Shenzhen 8th People's Hospital, Shenzhen, China.
Shenzhen Bao'an People's Hospital, Department of Orthopaedics and Traumatology, affiliated to Southern Medical University and Shenzhen 8th People's Hospital, Shenzhen, China and Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China.
出版信息
Bone Joint Res. 2017 Nov;6(11):612-618. doi: 10.1302/2046-3758.611.BJR-2017-0090.R1.
OBJECTIVES
This study looked to analyse the expression levels of microRNA-140-3p and microRNA-140-5p in synovial fluid, and their correlations to the severity of disease regarding knee osteoarthritis (OA).
METHODS
Knee joint synovial fluid samples were collected from 45 patients with OA of the knee (15 mild, 15 moderate and 15 severe), ten healthy volunteers, ten patients with gouty arthritis, and ten with rheumatoid arthritis. The Kellgren-Lawrence grading (KLG) was used to assess the radiological severity of knee OA, and the patients were stratified into mild (KLG < 2), moderate (KLG = 2), and severe (KLG > 2). The expression of miR-140-3p and miR-140-5p of individual samples was measured by SYBR Green quantitative polymerase chain reaction (PCR) analysis. The expression of miR-140-3p and miR-140-5p was normalised to U6 internal control using the 2 method. All data were processed using SPSS software.
RESULTS
Expression of both miR-140-3p and miR-140-5p was downregulated in OA synovial fluid, showing a statistical difference between the OA and non-OA group, and increased OA severity was associated with a decreased expression of miR-140-3p or miR-140-5p. The Spearman rank correlation analysis suggested that the expression of miR-140-3p or miR-140-5p was negatively correlated with OA severity. In addition, the expression of miR-140-5p was 7.4 times higher than that of miR-140-3p across all groups.
CONCLUSION
The dysregulation of miR-140-3p and miR-140-5p in synovial fluid and their correlations with the disease severity of OA may provide an important experimental basis for OA classification, and the miR-140-3p/miR-140-5p are of great potential as biomarkers in the diagnosis and clinical management of patients with OA.: C-M. Yin, W-C-W. Suen, S. Lin, X-M. Wu, G. Li, X-H. Pan. Dysregulation of both miR-140-3p and miR-140-5p in synovial fluid correlate with osteoarthritis severity. 2017;6:612-618. DOI: 10.1302/2046-3758.611.BJR-2017-0090.R1.
目的
本研究旨在分析滑液中微小RNA - 140 - 3p和微小RNA - 140 - 5p的表达水平，以及它们与膝关节骨关节炎（OA）疾病严重程度的相关性。
方法
收集45例膝关节OA患者（轻度15例、中度15例、重度15例）、10名健康志愿者、10例痛风性关节炎患者和10例类风湿关节炎患者的膝关节滑液样本。采用凯尔格伦 - 劳伦斯分级（KLG）评估膝关节OA的放射学严重程度，患者被分为轻度（KLG < 2）、中度（KLG = 2）和重度（KLG > 2）。通过SYBR Green定量聚合酶链反应（PCR）分析测量各个样本中miR - 140 - 3p和miR - 140 - 5p的表达。使用2−ΔΔCt方法将miR - 140 - 3p和miR - 140 - 5p的表达标准化为U6内参。所有数据使用SPSS软件进行处理。
结果
OA滑液中miR - 140 - 3p和miR - 140 - 5p的表达均下调，OA组与非OA组之间存在统计学差异，且OA严重程度增加与miR - 140 - 3p或miR - 140 - 5p表达降低相关。Spearman等级相关分析表明，miR - 140 - 3p或miR - 140 - 5p的表达与OA严重程度呈负相关。此外，所有组中miR - 140 - 5p的表达比miR - 140 - 3p高7.4倍。
结论
滑液中miR - 140 - 3p和miR - 140 - 5p的失调及其与OA疾病严重程度的相关性可能为OA分类提供重要的实验依据，且miR - 140 - 3p/miR - 140 - 5p作为OA患者诊断和临床管理中的生物标志物具有巨大潜力。：尹C - M，孙W - C - W，林S，吴X - M，李G，潘X - H。滑液中miR - 140 - 3p和miR - 140 - 5p的失调与骨关节炎严重程度相关。2017；6：612 - 618。DOI：10.1302/2046 - 3758.611.BJR - 2017 - 0090.R1。
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