Tóth Eszter Judit, Boros Éva, Hoffmann Alexandra, Szebenyi Csilla, Homa Mónika, Nagy Gábor, Vágvölgyi Csaba, Nagy István, Papp Tamás
MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary.
Faculty of Science and Informatics, Department of Microbiology, University of Szeged, Szeged, Hungary.
Front Immunol. 2017 Oct 18;8:1369. doi: 10.3389/fimmu.2017.01369. eCollection 2017.
Interaction of the human monocytic cell line, THP-1 with clinical isolates of three species were examined. Members of this filamentous fungal genus can cause deep mycoses emerging in both immunocompromised and immunocompetent patients. It was found that monocytes reacted only to the hyphal form of . Cells attached to the germ tubes and hyphae and production of elevated levels of interleukin (IL)-8 and IL-10 and a low level of TNF-α were measured. At the same time, monocytes failed to produce IL-6. This monocytic response, especially with the induction of the anti-inflammatory IL-10, correlates well to the observation that frequently cause chronic infections even in immunocompetent persons. Despite the attachment to the hyphae, monocytes could not reduce the viability of the fungus and the significant decrease in the relative transcript level of HLA-DRA assumes the lack of antigen presentation of the fungus by this cell type. and failed to induce the gathering of the cells or the production of any analyzed cytokines. Monocytes did not recognize conidia of species, even when melanin was lacking in their cell wall.
研究了人类单核细胞系THP-1与三种临床分离菌株的相互作用。该丝状真菌属的成员可导致免疫功能低下和免疫功能正常的患者出现深部真菌病。发现单核细胞仅对……的菌丝形式有反应。细胞附着于芽管和菌丝，并检测到白细胞介素（IL）-8和IL-10水平升高以及肿瘤坏死因子-α水平较低。同时，单核细胞未能产生IL-6。这种单核细胞反应，尤其是抗炎性IL-10的诱导，与……即使在免疫功能正常的人群中也常引起慢性感染的观察结果密切相关。尽管单核细胞附着于菌丝，但它们无法降低真菌的活力，并且HLA-DRA相对转录水平的显著降低表明这种细胞类型缺乏真菌的抗原呈递。……未能诱导细胞聚集或产生任何分析的细胞因子。单核细胞即使在其细胞壁中缺乏黑色素时也无法识别……种的分生孢子。
