Tóth Eszter Judit, Varga Mónika, Takó Miklós, Homa Mónika, Jáger Olivér, Hermesz Edit, Orvos Hajnalka, Nagy Gábor, Vágvölgyi Csaba, Papp Tamás
MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences-University of Szeged, 6726 Szeged, Hungary.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary.
Pathogens. 2020 Mar 21;9(3):235. doi: 10.3390/pathogens9030235.
is an ascomycete filamentous fungus causing local and invasive phaeohyphomycoses in both immunocompromised and immunocompetent patients. Neutrophils are crucial participants of the first line host defense against fungal infections. They migrate to the infected site and eliminate the infectious agents by various mechanisms including phagocytoses, oxidative damage, or formation of neutrophil extracellular trap (NET). Neutropenia may be a risk factor for phaeohyphomycoses, and restoration of the neutrophil function can improve the outcome of the infection. In the present study, interaction of primary human neutrophil granulocytes with the hyphae was examined and compared to that with the well characterized filamentous fungal pathogen . Neutrophils could recognize the serum opsonized hyphae of and attach to them. Myeloperoxidase release was also activated by a soluble factor present in the culture supernatant of the fungus. Induction of the oxidative burst was found to depend on serum opsonization of the hyphae. Although extracellular hydrogen peroxide production was induced, the fungus efficiently blocked the oxidative burst by acidifying the reaction environment. This blockage also affected the NET forming ability of the neutrophils.
是一种子囊菌丝状真菌，可在免疫功能低下和免疫功能正常的患者中引起局部和侵袭性暗色丝孢霉病。中性粒细胞是宿主抵御真菌感染的第一道防线的关键参与者。它们迁移到感染部位，并通过多种机制清除感染因子，包括吞噬作用、氧化损伤或形成中性粒细胞胞外陷阱（NET）。中性粒细胞减少可能是暗色丝孢霉病的一个危险因素，恢复中性粒细胞功能可改善感染结局。在本研究中，检测了原代人中性粒细胞与菌丝的相互作用，并将其与已充分表征的丝状真菌病原体的相互作用进行了比较。中性粒细胞能够识别经血清调理的的菌丝并附着于其上。真菌培养上清液中存在的一种可溶性因子也激活了髓过氧化物酶的释放。发现氧化爆发的诱导取决于菌丝的血清调理作用。虽然诱导了细胞外过氧化氢的产生，但真菌通过酸化反应环境有效地阻断了氧化爆发。这种阻断也影响了中性粒细胞形成NET的能力。
