伤口软膏（WO）的化学特性及其对骨折修复的影响：兔模型
Chemical characterization of wound ointment (WO) and its effects on fracture repair: a rabbit model.
作者信息
Ou Zhixue, Cheng Qi, Chen Yueping, Chen Tao, Rong Xiangbin, Long Feipan, Zhang Xiaoyun, Liang Qinghua, Feng Zhe
机构信息
Department of Traumatic Orthopedics and Hand Surgery, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Block B, No 10 Huadong Road, Nanning, 530011 Guangxi China.
Department of Traumatic Orthopedics, Daye Hospital of Traditional Chinese Medicine, Daye, 435100 Hubei China.
出版信息
Chin Med. 2017 Oct 30;12:31. doi: 10.1186/s13020-017-0152-y. eCollection 2017.
BACKGROUND
Wound ointment (WO), a kind of Chinese medicine, can significantly promote fracture healing. The study aimed at analyzing the chemical composition and the effects of WO on fracture of rabbits and tried to explore the corresponding molecular mechanism in cytokine.
METHODS
The qualitative and quantitative analysis of WO was conducted by liquid chromatography-mass spectrometry (LC-MS). Fifty-four Zealand mature male rabbits were randomly divided into 3 groups: Control group, Yunnan Baiyao (YB) group and WO group. All the rabbits suffered a fracture of right radius and were then stabilized with an external fixator. Treated with different methods, fracture healing was observed. The bone specimens were subjected to radiograph, immunohistochemistry (IHC) analysis, hematoxylin-eosin staining (HE), western blot and enzyme linked immunosorbent assay (ELISA).
RESULTS
A total of 12 active compositions were detected by LC-MS. Radiographs showed a considerably better bone healing and remodeling of the fracture in WO group. HE experiments showed that a large number of osteoclasts appeared in the early stage when treated with WO. In immunohistochemistry (IHC), western blot and ELISA test, significant increases in vascular endothelial growth factor (VEGF) expression were observed in WO group compared with other two groups.
CONCLUSIONS
Wound ointment contained active compositions which efficiently promoted fracture healing through increasing the expression of VEGF. Not applicable.
背景
创伤膏作为一种中药，能显著促进骨折愈合。本研究旨在分析创伤膏的化学成分及其对兔骨折的影响，并试图探索其在细胞因子方面的相应分子机制。
方法
采用液相色谱 - 质谱联用（LC - MS）对创伤膏进行定性和定量分析。将54只成年雄性新西兰兔随机分为3组：对照组、云南白药（YB）组和创伤膏组。所有兔子均造成右桡骨骨折，然后用外固定器固定。采用不同方法治疗后，观察骨折愈合情况。对骨标本进行X线摄影、免疫组织化学（IHC）分析、苏木精 - 伊红染色（HE）、蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）。
结果
通过LC - MS共检测到12种活性成分。X线摄影显示创伤膏组骨折的骨愈合和重塑明显更好。HE实验表明，创伤膏治疗早期出现大量破骨细胞。在免疫组织化学（IHC）、蛋白质免疫印迹法和ELISA检测中，与其他两组相比，创伤膏组血管内皮生长因子（VEGF）表达显著增加。
结论
创伤膏含有活性成分，通过增加VEGF的表达有效促进骨折愈合。不适用。
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