Palmer Jonathan C, Lord Megan S, Pinyon Jeremy L, Wise Andrew K, Lovell Nigel H, Carter Paul M, Enke Ya Lang, Housley Gary D, Green Rylie A
Graduate School of Biomedical Engineering, University of New South Wales Sydney, Sydney, New South Wales, Australia.
Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales Sydney, Sydney, New South Wales, Australia.
Laryngoscope. 2018 Jan;128(1):E47-E52. doi: 10.1002/lary.26885. Epub 2017 Nov 2.
OBJECTIVES/HYPOTHESIS: Biological components of perilymph affect the electrical performance of cochlear implants. Understanding the perilymph composition of common animal models will improve the understanding of this impact and improve the interpretation of results from animal studies and how it relates to humans.
Analysis and comparison of the proteomes of human, guinea pig, and cat perilymph.
Multiple perilymph samples from both guinea pigs and cats were analysed via liquid chromatography with tandem mass spectrometry. Proteins were identified using the Mascot database. Human data were obtained from a published dataset. Proteins identified were refined to form a proteome for each species.
Over 200 different proteins were found per species. There were 81, 39, and 64 proteins in the final human, guinea pig, and cat proteomes, respectively. Twenty-one proteins were common to all three species. Fifty-two percent of the cat proteome was found in the human proteome, and 31% of the guinea pig was common to human. The cat proteome had similar complexity to the human proteome in three protein classes, whereas the guinea pig had a similar complexity in two. The presence of albumin was significantly higher in human perilymph than in the other two species. Immunoglobulins were more abundant in the human than in the cat proteome.
Perilymph proteomes were compared across three species. The degree of crossover of proteins of both guinea pig and cat with human indicate that these animals suitable models for the human cochlea, albeit the cat perilymph is a closer match.
NA. Laryngoscope, 128:E47-E52, 2018.
目的/假设：外淋巴的生物成分会影响人工耳蜗的电性能。了解常见动物模型的外淋巴组成将有助于更好地理解这种影响，并改善对动物研究结果的解读以及其与人类的关系。
对人类、豚鼠和猫的外淋巴蛋白质组进行分析和比较。
通过液相色谱-串联质谱法分析豚鼠和猫的多个外淋巴样本。使用Mascot数据库鉴定蛋白质。人类数据来自已发表的数据集。对鉴定出的蛋白质进行细化，以形成每个物种的蛋白质组。
每个物种发现了200多种不同的蛋白质。最终的人类、豚鼠和猫蛋白质组中分别有81、39和64种蛋白质。所有三个物种共有21种蛋白质。猫蛋白质组的52%存在于人类蛋白质组中，豚鼠的31%与人类共有。猫蛋白质组在三类蛋白质中与人类蛋白质组具有相似的复杂性，而豚鼠在两类蛋白质中具有相似性。人类外淋巴中白蛋白的含量明显高于其他两个物种。免疫球蛋白在人类中比在猫蛋白质组中更丰富。
比较了三个物种的外淋巴蛋白质组。豚鼠和猫与人类的蛋白质交叉程度表明，这些动物是适合人类耳蜗的模型，尽管猫的外淋巴更接近人类。
无。《喉镜》，2018年，第128卷，E47-E52页。
