Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, South Korea.
Proteomics. 2021 Mar;21(5):e2000138. doi: 10.1002/pmic.202000138. Epub 2021 Feb 23.
The vast majority of sensorineural hearing loss is caused by impairment of the inner ear cells. Proteomic analysis of perilymph may therefore improve our understanding of inner ear diseases and hearing loss. However, the investigation of the human perilymph proteome was limited due to technical difficulties in perilymph sampling. The guinea pig (Cavia porcellus) is frequently used as an experimental model in preclinical hearing research. In this study, we analyzed samples of perilymph collected from 12 guinea pigs to overcome limited experimental information regarding its proteome. We identified a total of 1413 proteins, establishing a greatly expanded proteome of the previously inferred guinea pig perilymph. This provides a comprehensive proteomic resource for the research community, which will facilitate future molecular-phenotypic studies using the guinea pig as an experimental model of relevance to human inner ear biology.
绝大多数感音神经性听力损失是由内耳细胞损伤引起的。因此，外淋巴的蛋白质组学分析可以提高我们对内耳疾病和听力损失的认识。然而，由于在外淋巴取样方面存在技术困难，人类外淋巴蛋白质组的研究受到限制。豚鼠（Cavia porcellus）经常被用作临床前听力研究的实验模型。在这项研究中，我们分析了从 12 只豚鼠收集的外淋巴样本，以克服关于其蛋白质组的有限实验信息。我们总共鉴定出 1413 种蛋白质，建立了一个以前推断的豚鼠外淋巴蛋白质组的极大扩展。这为研究界提供了一个全面的蛋白质组资源，将有助于未来使用豚鼠作为与人类内耳生物学相关的实验模型进行分子表型研究。
