Khorrami Motahare, Haynes Paul A, Pastras Christopher, Asadnia Mohsen
School of Engineering, Macquarie University, Sydney, New South Wales, Australia.
School of Natural Sciences, Macquarie University, Sydney, New South Wales, Australia.
Proteomics. 2025 Jun 23;25(13):e13977. doi: 10.1002/pmic.13977.
The cochlea, an incredibly sensitive sensory system, detects sound waves and converts them into electrical signals the brain recognizes as sound. Damage to cochlear hair cells can release proteins, triggering biological responses that may impair hearing. Mass spectrometry-based proteomics offers insights into protein expression changes in cochlear tissues, improving our understanding of inner ear diseases. In this study, we performed a comprehensive proteomics analysis of whole cochlear tissue extracted from healthy guinea pigs and rats. The study optimized protein extraction protocols and analyzed cochlear protein expression using three biological replicates for each animal model. The results included the identification of 1841 proteins in guinea pigs and 3423 proteins in rats, with a high overlap in cochlear protein expression between the left and right ears-93% in guinea pigs and 89% in rats. The findings validate the assumption that the cochlear tissues from both sides of the ears can be considered biologically equivalent. This experiment provides a comprehensive cochlear proteome for guinea pigs and rats, supporting future studies on inner ear disorders.
耳蜗是一个极其敏感的感觉系统，它能检测声波并将其转化为大脑能够识别为声音的电信号。耳蜗毛细胞受损会释放蛋白质，引发可能损害听力的生物学反应。基于质谱的蛋白质组学有助于深入了解耳蜗组织中的蛋白质表达变化，增进我们对内耳疾病的认识。在本研究中，我们对从健康豚鼠和大鼠中提取的整个耳蜗组织进行了全面的蛋白质组学分析。该研究优化了蛋白质提取方案，并对每个动物模型使用三个生物学重复样本分析耳蜗蛋白质表达。结果在豚鼠中鉴定出1841种蛋白质，在大鼠中鉴定出3423种蛋白质，左右耳之间的耳蜗蛋白质表达有高度重叠——豚鼠中为93%，大鼠中为89%。这些发现验证了双耳两侧的耳蜗组织可被视为生物学上等效的这一假设。本实验为豚鼠和大鼠提供了一个全面的耳蜗蛋白质组，为未来内耳疾病的研究提供了支持。
