Johnson L P, Schlievert P M
Mol Gen Genet. 1984;194(1-2):52-6. doi: 10.1007/BF00383496.
The structural gene for group A streptococcal pyrogenic exotoxin (SPE) type A was cloned into E. coli. DNA fragments used for cloning the toxin gene were isolated from bacteriophage T12. Toxin, present in cell lysates of E. coli clones, immunoprecipitated with antisera raised against purified SPE type A and formed a line of identity with streptococcal-derived A toxin. The cloned toxin shared the following biological activities with streptococcal A toxin: Pyrogenicity; enhancement of host susceptibility to lethal endotoxin shock; nonspecific lymphocyte mitogenicity; and alteration of immunoglobulin production. The physical location of the toxin gene on the phage T12 genome was determined.
A群链球菌A型致热外毒素（SPE）的结构基因被克隆到大肠杆菌中。用于克隆毒素基因的DNA片段是从噬菌体T12中分离出来的。存在于大肠杆菌克隆细胞裂解物中的毒素，能用抗纯化A型SPE产生的抗血清进行免疫沉淀，并与链球菌来源的A型毒素形成一条同一线。克隆的毒素与链球菌A型毒素具有以下生物学活性：致热性；增强宿主对内毒素致死性休克的易感性；非特异性淋巴细胞有丝分裂原性；以及免疫球蛋白产生的改变。确定了毒素基因在噬菌体T12基因组上的物理位置。
