Saito T, Tanaka R, Kouno M, Washiyama K, Abe S, Kumanishi T
Department of Neurosurgery, Brain Research Institute, Niigata University, Japan.
J Neurosurg. 1989 Jan;70(1):81-5. doi: 10.3171/jns.1989.70.1.0081.
Subpopulations of tumor-infiltrating lymphocytes (TIL's) and the major histocompatibility complex (MHC) antigens of neoplastic cells were examined in three intracranial germinomas by an immunohistochemical method using monoclonal antibodies. About 70% to 80% of TIL's were T lymphocytes which were either infiltrating diffusely or in clusters, whereas 20% to 30% of TIL's were B lymphocytes which tend to cluster in tumor tissues. Examination of T lymphocyte phenotypes revealed both the cytotoxic/suppressor and helper/inducer T lymphocytes, as in other tumors. However, the existence of a considerable number of B lymphocytes in the TIL population was uncommon and seemed to be a characteristic feature of the intracranial germinoma, which might suggest a difference of host immune response to this neoplasm as compared to other tumors. On examination of the MHC antigens, no MHC class I or II antigens in the neoplastic cells were stained, while positive staining for both antigens was seen in the TIL and stroma tissues. From these findings, it was suggested that the degree of TIL infiltration might not be correlated with the expression of MHC antigens in neoplastic cells in cases of primary intracranial germinoma.
采用单克隆抗体免疫组织化学方法，对3例颅内生殖细胞瘤中的肿瘤浸润淋巴细胞（TIL）亚群和肿瘤细胞的主要组织相容性复合体（MHC）抗原进行了检测。约70%至80%的TIL为T淋巴细胞，呈弥漫性或簇状浸润，而20%至30%的TIL为B淋巴细胞，倾向于在肿瘤组织中聚集。T淋巴细胞表型检测显示，与其他肿瘤一样，存在细胞毒性/抑制性T淋巴细胞和辅助/诱导性T淋巴细胞。然而，TIL群体中存在相当数量的B淋巴细胞并不常见，似乎是颅内生殖细胞瘤的一个特征，这可能表明与其他肿瘤相比，宿主对该肿瘤的免疫反应存在差异。对MHC抗原进行检测时，肿瘤细胞中未检测到MHCⅠ类或Ⅱ类抗原染色，而在TIL和基质组织中均可见两种抗原的阳性染色。从这些发现推测，在原发性颅内生殖细胞瘤病例中，TIL浸润程度可能与肿瘤细胞中MHC抗原的表达无关。
