Dietl J, Horny H P, Ruck P, Kaiserling E
Department of Obstetrics and Gynecology, University of Tübingen, Germany.
Cancer. 1993 Apr 15;71(8):2562-8. doi: 10.1002/1097-0142(19930415)71:8<2562::aid-cncr2820710821>3.0.co;2-e.
Human neoplasms often are accompanied by an inflammatory infiltrate. It has been proposed that this represents an immunologic response to the tumor. Dysgerminoma, a germ cell tumor of the ovary, is a classic example of this phenomenon. The authors investigated the immunophenotype of the tumor-infiltrating lymphoreticular cells (TIL) and tumor cells in this rare malignancy.
Tissue from seven dysgerminomas of the ovary was fixed in formaldehyde solution and embedded in paraffin and investigated immunohistochemically with a broad panel of monoclonal antibodies. In one case, additional immunohistochemical investigations were performed on cryopreserved tumor tissue.
All seven tumors showed a marked cellular stromal reaction with formation of disseminated granulomas similar to that seen in the closely related testicular seminoma. The TIL were preponderantly T-cells (CD43+, CD45RO+, OPD4+) and macrophages/epithelioid cells (MAC387+, CD68+), B-cells (CD20+, Ki-B3+), natural killer cells (CD57+), and immune-accessory cells (CD1+, CD35+) were rare in most cases. In the one case in which cryopreserved tissue was available, most of the intratumoral T-cells belonged to the CD8+ (cytotoxic/suppressor) subtype, and most of the intratumoral T-cells expressed the alpha/beta heterodimer of the T-cell antigen receptor; gamma/delta + T-cells were exceedingly rare. Some of the macrophages/epithelioid cells were found to express activation antigens (interleukin-2 receptor, transferrin receptor, HLA-DR2). Antibodies against placental alkaline phosphatase and pancytokeratin each stained tumor cells in six cases. Virtually no tumor cells were found to express major histocompatibility complex (MHC) Class II antigens.
The immunohistochemical findings concerning the tumor cells and TIL in dysgerminoma of the ovary provide additional evidence of a close relation to seminoma of the testis.
人类肿瘤常伴有炎性浸润。有人提出这代表了对肿瘤的免疫反应。无性细胞瘤是卵巢的一种生殖细胞肿瘤，是这种现象的典型例子。作者研究了这种罕见恶性肿瘤中肿瘤浸润性淋巴网状细胞（TIL）和肿瘤细胞的免疫表型。
取自7例卵巢无性细胞瘤的组织用甲醛溶液固定，石蜡包埋，并用一组广泛的单克隆抗体进行免疫组织化学研究。在1例中，对冷冻保存的肿瘤组织进行了额外的免疫组织化学研究。
所有7例肿瘤均表现出明显的细胞性间质反应，形成散在的肉芽肿，类似于在密切相关的睾丸精原细胞瘤中所见。TIL主要为T细胞（CD43 +、CD45RO +、OPD4 +）和巨噬细胞/上皮样细胞（MAC387 +、CD68 +），B细胞（CD20 +、Ki - B3 +）、自然杀伤细胞（CD57 +）和免疫辅助细胞（CD1 +、CD35 +）在大多数情况下很少见。在有冷冻保存组织的1例中，大多数瘤内T细胞属于CD8 +（细胞毒性/抑制性）亚型，大多数瘤内T细胞表达T细胞抗原受体的α/β异二聚体；γ/δ + T细胞极其罕见。一些巨噬细胞/上皮样细胞被发现表达激活抗原（白细胞介素-2受体、转铁蛋白受体、HLA - DR2）。抗胎盘碱性磷酸酶和全细胞角蛋白的抗体在6例中均染色肿瘤细胞。几乎没有发现肿瘤细胞表达主要组织相容性复合体（MHC）II类抗原。
关于卵巢无性细胞瘤中肿瘤细胞和TIL的免疫组织化学发现为其与睾丸精原细胞瘤的密切关系提供了额外证据。
