School of Marine Science, Ningbo University, Ningbo, 315211, China.
Department of Food Science, Cornell University, Ithaca, New York, NY, USA.
Appl Microbiol Biotechnol. 2018 Jan;102(1):355-366. doi: 10.1007/s00253-017-8601-1. Epub 2017 Nov 2.
Low-dose (LD, 100 mg kg day), moderate-dose (MD, 200 mg kg day), and high-dose (HD, 600 mg kg day) krill oil treatments have a stepwise, enhanced effect on alleviating hyperlipidemia, and 16S rRNA sequencing of the fecal samples demonstrates that krill oil treatment alters microbial communities. Feces may not represent all microbial communities in the gastrointestinal (GI) tract. Therefore, in this study, the stored ileal and colon samples collected from LD and HD groups were sequenced, and the location-specific modulations of microbial communities were observed after krill oil treatments. The 16S rRNA sequencing of the ileal samples showed that the LD and HD groups have similar patterns between control and high-fat diet (HFD) treatments, and six most abundant genera and 40 operational taxonomic units that respond to krill oil treatment were identified. However, the 16S rRNA sequencing of the colon samples showed that LD krill oil shifts the structure from the HFD to that of the control, whereas the HD group was distributed between the control and HFD groups. The corresponding most abundant genera and responsive OTUs totaled 4 and 45, respectively. In conclusion, different gastrointestinal tract locations contain different microbial communities. These results will help to provide a comprehensive understanding of the role of dietary krill oil in modulating the gut microbiota and alleviating hyperlipidemia.
低剂量（LD，100mg/kg 天）、中剂量（MD，200mg/kg 天）和高剂量（HD，600mg/kg 天）磷虾油处理对缓解高血脂症有逐步增强的效果，粪便 16S rRNA 测序表明磷虾油处理改变了微生物群落。粪便可能不能代表胃肠道（GI）中的所有微生物群落。因此，在这项研究中，对 LD 和 HD 组中储存的回肠和结肠样本进行了测序，并观察了磷虾油处理后微生物群落的特定部位调节。回肠样本的 16S rRNA 测序表明，LD 和 HD 组在对照和高脂肪饮食（HFD）处理之间具有相似的模式，并且鉴定了 6 个最丰富的属和 40 个对磷虾油处理有反应的分类操作单元。然而，结肠样本的 16S rRNA 测序表明，LD 磷虾油将结构从 HFD 转变为对照，而 HD 组分布在对照和 HFD 组之间。相应的最丰富的属和响应 OTU 总数分别为 4 和 45。总之，不同的胃肠道部位含有不同的微生物群落。这些结果将有助于全面了解饮食磷虾油在调节肠道微生物群和缓解高血脂症中的作用。
