Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu, People's Republic of China.
Department of Neurosurgery, Children's Hospital of Soochow University, Suzhou, 215000, Jiangsu, People's Republic of China.
Cell Mol Neurobiol. 2018 May;38(4):861-868. doi: 10.1007/s10571-017-0560-7. Epub 2017 Nov 2.
Survivin is a tumor-associated gene, which has been detected in a wide variety of human tumors. Previous research has shown that Survivin can affect hepatoma carcinoma cell radiosensitivity. However, little is known about the role of Survivin in ionizing radiation resistance in glioma cells. In this study, we aimed to identify the effects of Survivin on ionizing radiation resistance in glioma cell line T98. Our results showed that downregulation of Survivin gene expression and ionizing irradiation could both inhibit T98 cell proliferation by assays in vitro including CCK-8 and immunohistochemistry. The inhibitory effect of downregulation of Survivin combined with irradiation was the most significant compared with other groups. Results of Western blotting and flow cytometric analysis also showed that downregulation of Survivin combined with the irradiation group achieved the highest apoptosis rate. Experimental results in vivo by intracranial implanting into nude mice were consistent with those in vitro. These findings indicated that ionizing radiation resistance of human T98 glioma cells can be inhibited effectively after Survivin gene silencing.
Survivin 是一种肿瘤相关基因，已在多种人类肿瘤中被检测到。先前的研究表明 Survivin 可以影响肝癌细胞的放射敏感性。然而，关于 Survivin 在胶质瘤细胞中对电离辐射抵抗的作用知之甚少。在这项研究中，我们旨在确定 Survivin 对胶质瘤细胞系 T98 电离辐射抵抗的影响。我们的结果表明，下调 Survivin 基因表达和电离照射均可通过体外 CCK-8 和免疫组织化学检测抑制 T98 细胞增殖。与其他组相比，下调 Survivin 与照射联合的抑制作用最显著。Western blot 和流式细胞术分析的结果也表明，下调 Survivin 与照射联合组的凋亡率最高。裸鼠颅内植入的体内实验结果与体外结果一致。这些发现表明，沉默 Survivin 基因后可有效抑制人 T98 神经胶质瘤细胞的电离辐射抵抗。
