核糖体与真核生物信使核糖核酸5'-末端前导序列的结合。
Binding of ribosomes to 5'-terminal leader sequences of eukaryotic messenger RNAs.
作者信息
Filipowicz W, Haenni A L
出版信息
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3111-5. doi: 10.1073/pnas.76.7.3111.
Abstract
Tobacco mosaic virus and turnip yellow mosaic virus genome RNAs, which have long (greater than or equal to 69 nucleotides) 5'-terminal leader sequences preceding the AUG initiation codon, can form disome initiation complexes when incubated with a wheat germ extract and sparsomycin. In tobacco mosaic virus RNA, whereas one ribosome in such complexes presumably occupies the AUG-containing initiation site, the other is located within the RNA leader sequence. The results favor a model of eukaryotic initiation in which ribosomes first bind to the 5' end of the mRNA independently of AUG, then advance to the AUG initiation codon, where protein synthesis ensues.
摘要
烟草花叶病毒和芜菁黄花叶病毒基因组RNA在AUG起始密码子之前有长（大于或等于69个核苷酸）的5'末端前导序列，当与小麦胚芽提取物和稀疏霉素一起孵育时，能形成二核糖体起始复合物。在烟草花叶病毒RNA中，这种复合物中的一个核糖体大概占据含AUG的起始位点，另一个则位于RNA前导序列内。这些结果支持一种真核生物起始模型，即核糖体首先独立于AUG结合到mRNA的5'末端，然后前进到AUG起始密码子，在那里开始蛋白质合成。
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