Pinck M, Fritsch C, Ravelonandro M, Thivent C, Pinck L
Nucleic Acids Res. 1981 Mar 11;9(5):1087-100. doi: 10.1093/nar/9.5.1087.
RNA 3 of alfalfa mosaic virus (AlMV) contains information for two genes: near the 5' end an active gene coding for a 35 Kd protein and, near the 3' end, a silent gene coding for viral coat protein. We have determined a sequence of 318 nucleotides which contains the potential initiation codon for the 35 Kd protein at 258 nucleotides from the 5' end. This long leader sequence can form initiation complexes containing three 80 S ribosomes. A shorter species of RNA, corresponding to a molecule of RNA 3 lacking the cap and the first 154 nucleotides (RNA 3') has been isolated. The remaining leader sequence of 104 nucleotides in RNA 3' forms a single 80 S initiation complex with wheat germ ribosomes. The location of the regions of the leader sequence of RNA 3 involved in initiation complex formation with 80 S ribosomes is reported.
苜蓿花叶病毒（AlMV）的RNA 3包含两个基因的信息：在5'端附近是一个编码35 Kd蛋白的活性基因，在3'端附近是一个编码病毒外壳蛋白的沉默基因。我们确定了一段318个核苷酸的序列，该序列在距5'端258个核苷酸处包含35 Kd蛋白的潜在起始密码子。这个长的前导序列可以形成包含三个80 S核糖体的起始复合物。已经分离出一种较短的RNA种类，它对应于缺少帽和前154个核苷酸的RNA 3分子（RNA 3'）。RNA 3'中剩余的104个核苷酸的前导序列与小麦胚芽核糖体形成单个80 S起始复合物。报道了RNA 3前导序列中与80 S核糖体形成起始复合物相关区域的位置。
