一种使用铂纳米颗粒对皮摩尔水平的Ag⁺进行超灵敏比色检测的简单测定法。
A Simple Assay for Ultrasensitive Colorimetric Detection of Ag⁺ at Picomolar Levels Using Platinum Nanoparticles.
作者信息
Wang Yi-Wei, Wang Meili, Wang Lixing, Xu Hui, Tang Shurong, Yang Huang-Hao, Zhang Lan, Song Hongbo
机构信息
Key Laboratory of Predictive Microbiology and Chemical Residual Analysis, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350108, China.
出版信息
Sensors (Basel). 2017 Nov 2;17(11):2521. doi: 10.3390/s17112521.
In this work, uniformly-dispersed platinum nanoparticles (PtNPs) were synthesized by a simple chemical reduction method, in which citric acid and sodium borohydride acted as a stabilizer and reducer, respectively. An ultrasensitive colorimetric sensor for the facile and rapid detection of Ag⁺ ions was constructed based on the peroxidase mimetic activities of the obtained PtNPs, which can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ to produce colored products. The introduced Ag⁺ would be reduced to Ag⁰ by the capped citric acid, and the deposition of Ag⁰ on the PtNPs surface, can effectively inhibit the peroxidase-mimetic activity of PtNPs. Through measuring the maximum absorption signal of oxidized TMB at 652 nm, ultra-low detection limits (7.8 pM) of Ag⁺ can be reached. In addition to such high sensitivity, the colorimetric assay also displays excellent selectivity for other ions of interest and shows great potential for the detection of Ag⁺ in real water samples.
在本工作中，通过简单的化学还原法合成了均匀分散的铂纳米颗粒（PtNPs），其中柠檬酸和硼氢化钠分别作为稳定剂和还原剂。基于所制备的PtNPs的过氧化物酶模拟活性构建了一种用于简便快速检测Ag⁺离子的超灵敏比色传感器，该传感器可催化H₂O₂氧化3,3',5,5'-四甲基联苯胺（TMB）生成有色产物。引入的Ag⁺会被封端的柠檬酸还原为Ag⁰，并且Ag⁰在PtNPs表面的沉积能够有效抑制PtNPs的过氧化物酶模拟活性。通过测量氧化态TMB在652 nm处的最大吸收信号，可实现Ag⁺的超低检测限（7.8 pM）。除了具有如此高的灵敏度外，该比色测定法对其他感兴趣的离子还表现出优异的选择性，并且在实际水样中检测Ag⁺方面显示出巨大潜力。
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