Garg Mayank, Vishwakarma Neelam, Sharma Amit L, Mizaikoff Boris, Singh Suman
CSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
ACS Omega. 2020 Jan 21;5(4):1927-1937. doi: 10.1021/acsomega.9b03655. eCollection 2020 Feb 4.
The color generating from the biochemical reaction between 3,3',5,5'-tetramethylbenzidine and Lysine@WS QDs was used a signal for the detection of hydrogen peroxide. The QDs were prepared using a combination of techniques, that is, probe sonication and hydrothermal treatment. Analysis via UV-vis spectroscopy, Fourier transform infrared and Raman spectroscopy, X-ray diffraction, energy-dispersive spectroscopy, and transmission electron microscopy yielded detailed information on the nature and characteristics of these quantum dots. Furthermore, as-synthesized quantum dots were studied for their capability to mimic peroxidase enzyme using 3,3',5,5'-tetramethylbenzidine as a substrate. Consequently, a colorimetric sensor utilizing Lysine@WS QDs could detect hydrogen peroxide in a range of 0.1-60 μM with a response time of 5 min. The same material was used for HO detection using impedance spectroscopy, which yielded a dynamic range of 0.1-350 μM with a response time of 30-40 s.
3,3',5,5'-四甲基联苯胺与赖氨酸@WS量子点之间的生化反应产生的颜色被用作检测过氧化氢的信号。量子点采用多种技术相结合的方法制备，即探针超声处理和水热处理。通过紫外-可见光谱、傅里叶变换红外光谱和拉曼光谱、X射线衍射、能量色散光谱和透射电子显微镜进行分析，得到了这些量子点的性质和特征的详细信息。此外，以3,3',5,5'-四甲基联苯胺为底物，研究了合成的量子点模拟过氧化物酶的能力。因此，利用赖氨酸@WS量子点的比色传感器能够在0.1-60μM范围内检测过氧化氢，响应时间为5分钟。使用阻抗谱对相同材料进行HO检测，其动态范围为0.1-350μM，响应时间为30-40秒。
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