Department of Neurosurgery, University Hospitals Leuven, KU Leuven, Herestraat 49, 3000 Leuven, Belgium; Department of Neuroscience, Laboratory for Experimental Neurosurgery and Neuroanatomy, University Hospitals Leuven, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
Behav Brain Res. 2019 May 17;364:457-463. doi: 10.1016/j.bbr.2017.10.035. Epub 2017 Nov 1.
Stereotaxy is frequently employed in rodent models for precise brain injections or implantations. We intended to map the current practice in rat stereotaxy and list proposals to improve targeting accuracy.
We selected 235 publications on rat stereotaxy from the last 5 years. We collected data on the subjects, targets, coordinate system used, postoperative implant verification and analysis of subjects with off-target implants.
Approximately 10,000 rats, mostly Sprague-Dawley and Wistar, were subjected to stereotaxy. Although referenced in 57% of the studies, only 10% of the subjects resembled those used in the Paxinos atlas. Popular procedures were injections (62%) and implantation of cannulas (20%) and electrodes (8%). Right-sided and bilateral targets were more often used than left-sided targets. Bregma served as a stereotaxic origin in 96% of the publications, although for 27% of the targets, the entry was closer to lambda. The Euclidian distance from the target to the interaural line midpoint and to lambda was shorter than to bregma in 38% and 5% of the cases, respectively. Bregma and the dura/brain surface were the most popular dorsoventral References Implantation accuracy was assessed almost exclusively histologically. 39% of the studies did not perform any accuracy check. The number of on-target implants was reported in only 8% of the studies. Only 15% of the publications reported that they excluded subjects with off-target implants.
Although well-established, rat stereotaxy could be improved, mainly in acquiring target coordinates, selecting a stereotaxic origin and verifying and reporting accuracy.
立体定位技术常用于精确的脑内注射或植入手术。我们旨在描述当前大鼠立体定位的实践情况，并提出提高靶向准确性的建议。
我们从过去 5 年中选择了 235 篇关于大鼠立体定位的出版物。我们收集了有关实验对象、目标、使用的坐标系、术后植入物验证以及分析非目标植入物实验对象的数据。
大约有 10000 只大鼠（主要是 Sprague-Dawley 和 Wistar 大鼠）接受了立体定位手术。尽管 57%的研究都提到了参考坐标，但只有 10%的实验对象与 Paxinos 图谱中的大鼠相似。常用的手术方法是注射（62%）和植入套管（20%）和电极（8%）。右侧和双侧目标比左侧目标更常用。96%的出版物都以 Bregma 作为立体定位的原点，尽管对于 27%的目标，入点更靠近 lambda。从目标到耳间线中点和 lambda 的欧几里得距离比到 Bregma 的距离短，分别占 38%和 5%。Bregma 和硬脑膜/脑表面是最常用的背-腹参考。植入物的准确性几乎完全通过组织学评估。39%的研究没有进行任何准确性检查。只有 8%的研究报告了目标植入物的数量。只有 15%的出版物报告称他们排除了非目标植入物的实验对象。
尽管大鼠立体定位技术已经得到了很好的建立，但仍有改进的空间，主要是在获取目标坐标、选择立体定位原点以及验证和报告准确性方面。
