Department of Biosciences, Swansea University, Swansea, Wales.
Swansea University Medical School, Swansea University, Swansea, Wales.
Appl Environ Microbiol. 2018 Jan 2;84(2). doi: 10.1128/AEM.01401-17. Print 2018 Jan 15.
The aim of the current work was to identify key features of the fungal proteome involved in the active decay of beechwood blocks by the white rot fungus at 20°C and 24°C. A combination of protein and domain analyses ensured a high level of annotation, which revealed that while the variation in the proteins identified was high between replicates, there was a considerable degree of functional conservation between the two temperatures. Further analysis revealed differences in the pathways and processes employed by the fungus at the different temperatures, particularly in relation to nutrient acquisition and xenobiotic mitigation. Key features showing temperature-dependent variation in mechanisms for both lignocellulose decomposition and sugar utilization were found, alongside differences in the enzymes involved in mitigation against damage caused by toxic phenolic compounds and oxidative stress. This work was conducted using the wood decay fungus , grown on solid wood blocks to closely mimic the natural environment, and gives greater insight into the proteome of an important environmental fungus during active decay. We show that a change in incubation temperature from 20°C to 24°C altered the protein profile. Proteomic studies in the field of white-rotting basidiomycetes have thus far been hampered by poor annotation of protein databases, with a large proportion of proteins simply with unknown function. This study was enhanced by extensive protein domain analysis, enabling a higher level of functional assignment and greater understanding of the proteome composition. This work revealed a strong interdependence of the primary process of nutrient acquisition and specialized metabolic processes for the detoxification of plant extractives and the phenolic breakdown products of lignocellulose.
本研究旨在鉴定白腐真菌在 20°C 和 24°C 条件下主动腐朽山毛榉木块过程中涉及的真菌蛋白质组的关键特征。通过蛋白质和结构域分析的结合，确保了高度注释，结果表明，虽然在两个温度条件下，鉴定的蛋白质之间存在高度的变异性，但在功能上存在相当程度的保守性。进一步的分析揭示了真菌在不同温度下使用的途径和过程的差异，特别是在营养物质获取和异生物质缓解方面。发现了木质纤维素分解和糖利用机制中与温度相关的关键特征存在差异，同时也发现了参与缓解有毒酚类化合物和氧化应激造成的损伤的酶的差异。本研究使用固体木块上生长的木材腐朽真菌进行，这与自然环境非常相似，使我们对重要环境真菌在主动腐朽过程中的蛋白质组有了更深入的了解。我们表明，从 20°C 到 24°C 的孵育温度变化改变了蛋白质图谱。到目前为止，白腐担子菌领域的蛋白质组学研究一直受到蛋白质数据库注释不足的阻碍，其中很大一部分蛋白质的功能仍然未知。通过广泛的蛋白质结构域分析，本研究得到了增强，从而实现了更高水平的功能分配和对蛋白质组组成的更深入理解。这项工作揭示了营养物质获取的主要过程与专门的代谢过程之间的强烈相互依赖性，专门的代谢过程用于植物提取物的解毒和木质纤维素酚类分解产物的解毒。
