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两种α分病毒共同感染植物病原真菌立枯丝核菌的一个分离株。

Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani.

作者信息

Lyu Ruiling, Zhang Yi, Tang Qing, Li Yangyi, Cheng Jiasen, Fu Yanping, Chen Tao, Jiang Daohong, Xie Jiatao

机构信息

State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Xiangyang Academy of Agricultural Sciences, Xiangyang, 441057, Hubei, China.

出版信息

Arch Virol. 2018 Feb;163(2):515-520. doi: 10.1007/s00705-017-3627-3. Epub 2017 Nov 3.

DOI:10.1007/s00705-017-3627-3
PMID:29101540
Abstract

Seven dsRNA segments were detected from a single Rhizoctonia solani strain HG81. From the full-length cDNA sequences of four smaller dsRNA segments, the genomes of two related partitiviruses, designated as Rhizoctonia solani partitivirus 3 (RsPV3) and RsPV4, were determined. The genomes of RsPV3 and RsPV4 are both composed of two separate dsRNA segments, with each segment possessing a single open reading frame (ORF). ORF1 from RsPV3 and RsPV4 encodes a putative RNA-dependent RNA polymerase, while ORF2 of RsPV3 and RsPV4 encodes a putative capsid protein. RsPV3 and RsPV4 share high sequence identity with viruses classified within the genus Alphapartitivirus, family Partitiviridae.

摘要

从单一立枯丝核菌菌株HG81中检测到7条双链RNA片段。根据4条较小双链RNA片段的全长cDNA序列,确定了两种相关的分体病毒的基因组,分别命名为立枯丝核菌分体病毒3(RsPV3)和RsPV4。RsPV3和RsPV4的基因组均由两个独立的双链RNA片段组成,每个片段都有一个单一的开放阅读框(ORF)。RsPV3和RsPV4的ORF1编码一个假定的RNA依赖性RNA聚合酶,而RsPV3和RsPV4的ORF2编码一个假定的衣壳蛋白。RsPV3和RsPV4与分病毒科α-分病毒属内分类的病毒具有高度的序列同一性。

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