两种新型线粒体病毒共同感染引起小麦根腐病的单株拟禾本科镰孢菌的特性分析
Characterization of two novel mitoviruses co infecting a single strain of Fusarium pseudograminearum causing wheat crown rot.
作者信息
Pan Xin, Xie Yuan, Yan Shuwei, Zhang Xiaoting, Liu Xinxin, Jiao Youzhou, Gao Fei
机构信息
College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.
College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.
出版信息
Arch Virol. 2025 Mar 19;170(4):83. doi: 10.1007/s00705-025-06242-4.
Wheat crown rot (WCR), caused by Fusarium pseudograminearum, poses a threat to wheat production worldwide. Two mycoviruses, designated as "Fusarium pseudograminearum mitovirus 2" (FupgMV2) and "Fusarium pseudograminearum mitovirus 3" (FupgMV3), were identified in F. pseudograminearum strain YY514-10-2. The two viral genomes are 2,429 and 2,450 nucleotides long, each containing a single open reading frame (ORF) encoding a 724-amino-acid-long RNA-dependent RNA polymerase (RdRp), with 31.98% amino acid sequence identity to each other. BLASTp analysis revealed that the RdRp of FupgMV2 exhibits at least 88.84% amino acid sequence identity to that of Fusarium mitovirus 1, while the RdRp of FupgMV3 shows 80.19% amino acid sequence identity to that of Fusarium mitovirus 2. Phylogenetic analysis indicated that FupgMV2 and FupgMV3 both belong to the genus Unuamitovirus of the family Mitoviridae. This is the first report of two mitoviruses hosted by the same strain of the plant-pathogenic fungus F. pseudograminearum.
由禾谷镰刀菌引起的小麦根腐病对全球小麦生产构成威胁。在禾谷镰刀菌菌株YY514-10-2中鉴定出两种真菌病毒，分别命名为“禾谷镰刀菌线粒体病毒2”（FupgMV2）和“禾谷镰刀菌线粒体病毒3”（FupgMV3）。这两种病毒基因组长度分别为2429和2450个核苷酸，各自包含一个单一的开放阅读框（ORF），编码一个724个氨基酸长的RNA依赖的RNA聚合酶（RdRp），彼此之间氨基酸序列同一性为31.98%。BLASTp分析表明，FupgMV2的RdRp与镰刀菌线粒体病毒1的RdRp氨基酸序列同一性至少为88.84%，而FupgMV3的RdRp与镰刀菌线粒体病毒2的RdRp氨基酸序列同一性为80.19%。系统发育分析表明，FupgMV2和FupgMV3均属于线粒体病毒科的Unuamitovirus属。这是首次报道同一株植物病原真菌禾谷镰刀菌携带两种线粒体病毒。
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