Alizadeh Javad, Shojaei Shahla, Sepanjnia Adel, Hashemi Mohammad, Eftekharpour Eftekhar, Ghavami Saeid
Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2.
Department of Immunology, School of Medicine, Jiroft University of Medical Science, Jiroft, Iran.
Methods Mol Biol. 2019;1854:87-103. doi: 10.1007/7651_2017_84.
Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged sword in cancer biology which either promotes oncogenesis/invasion or sensitizes the tumor cells to chemotherapy induced apoptosis. Recent investigations have provided direct evidence for regulation of cellular phenotype via autophagy pathway. One of the most important types of phenotype conversion is Epithelial-Mesenchymal-Transition (EMT), resulting in alteration of epithelial cell properties to a more mesenchymal form. In the current chapter, we provide a method which is established and being used in our laboratory for detection of autophagy and EMT in lung epithelial cells and show the involvement of autophagy in modulation of cellular phenotype.
自噬在从细胞增殖到细胞分裂、迁移和分化等许多细胞活动中日益被视为核心参与者。然而，在癌症生物学中它也被视为一把双刃剑，既能促进肿瘤发生/侵袭，也能使肿瘤细胞对化疗诱导的凋亡敏感。最近的研究为通过自噬途径调节细胞表型提供了直接证据。最重要的表型转化类型之一是上皮-间质转化（EMT），导致上皮细胞特性改变为更具间充质的形式。在本章中，我们提供了一种在我们实验室已建立并正在使用的方法，用于检测肺上皮细胞中的自噬和EMT，并展示自噬在调节细胞表型中的作用。
