Varlamova E G, Goltyaev M V, Novoselov V I, Fesenko E E
Institute of Cell Biophysics, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia.
Dokl Biochem Biophys. 2017 Sep;476(1):320-322. doi: 10.1134/S160767291705012X. Epub 2017 Nov 4.
The intracellular localization of human selenoprotein SelI and the degree of expression of its gene in different human tumor cell lines were determined. It was found that the SelI protein is present in the nucleus, cytoplasm, and endoplasmic reticulum and is absent in the nucleolus. Since the oxidative stress caused by a sharp increase in the content of free radicals in the body is one of the causes of malignant transformation, the study of the role of the trace element selenium and selenocysteine-containing proteins as antioxidants in carcinogenesis is of great scientific interest.
测定了人类硒蛋白SelI在细胞内的定位及其基因在不同人类肿瘤细胞系中的表达程度。结果发现，SelI蛋白存在于细胞核、细胞质和内质网中，而核仁中不存在。由于体内自由基含量急剧增加所引起的氧化应激是恶性转化的原因之一，因此研究微量元素硒和含硒半胱氨酸的蛋白质作为抗氧化剂在致癌过程中的作用具有重大的科学意义。
