Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea.
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Jeollanam-do 58245, Korea.
Nutrients. 2018 Dec 10;10(12):1952. doi: 10.3390/nu10121952.
Bojungikgi-tang (BJIGT; Bu Zhong Yi Qi Tang in China, Hochuekkito in Japan) is a traditional Oriental herbal formula comprised of eight medicinal herbs that has long been used for the treatment of digestive disorders. A recent clinical study from South Korea reported that BJIGT-gamibang administration may be effective in treating dementia. We aimed to establish scientific evidence for the anti-dementia effects of BJIGT using in vitro and in vivo experimental models. We measured amyloid- β (Aβ) aggregation, β-secretase (BACE), and antioxidant activity in a cell free system. Neuroprotective effects were assessed using CCK-8. Imprinting control region (ICR) mice were divided into the following six groups: Normal control, Aβ-injected, Aβ-injection + oral BJIGT gavage (200, 400, or 800 mg/kg/day), and Aβ-injection + oral morin administration (10 mg/kg/day). Subsequently, behavioral evaluations were conducted and brain samples were collected from all the animals and assessed. BJIGT enhanced inhibition of Aβ aggregation and BACE activity in vivo, as well as antioxidant activity in in vitro, cell-free systems. BJIGT also exerted neuroprotective effects in a hydroperoxide (H₂O₂)-induced damaged HT22 hippocampal cell line model. In addition, BJIGT administration significantly ameliorated cognitive impairments in Aβ-injected mice, as assessed by the passive avoidance and Y-maze tests. Furthermore, BJIGT treatment suppressed Aβ aggregation and expression, as well as expression of Aβ, NeuN, and brain-derived neurotrophic factor (BDNF) in the hippocampi of Aβ-injected mice. Overall, our results demonstrate that, with further testing in clinical populations, BJIGT may have great utility for the treatment of dementia and especially Alzheimer's disease.
补阳还五汤（BJIGT；在中国称为补中益气汤，在日本称为 Hochuekkito）是一种由八种草药组成的传统东方草药配方，长期以来一直用于治疗消化系统疾病。最近来自韩国的一项临床研究报告称，BJIGT-甘比邦加（gamibang）给药可能对治疗痴呆症有效。我们旨在使用体外和体内实验模型为 BJIGT 的抗痴呆作用建立科学证据。我们在无细胞系统中测量了淀粉样蛋白-β（Aβ）聚集、β-分泌酶（BACE）和抗氧化活性。使用 CCK-8 评估神经保护作用。印迹控制区（ICR）小鼠分为以下六组：正常对照组、Aβ 注射组、Aβ 注射+口服 BJIGT 灌胃（200、400 或 800 mg/kg/天）组和 Aβ 注射+口服桑色素给药（10 mg/kg/天）组。随后对所有动物进行行为评估并收集大脑样本进行评估。BJIGT 增强了体内 Aβ 聚集和 BACE 活性的抑制作用，以及体外无细胞系统中的抗氧化活性。BJIGT 还在过氧化氢（H₂O₂）诱导的受损 HT22 海马细胞系模型中发挥了神经保护作用。此外，BJIGT 给药显著改善了 Aβ 注射小鼠的认知障碍，通过被动回避和 Y 迷宫测试进行评估。此外，BJIGT 治疗抑制了 Aβ 聚集和表达，以及 Aβ、NeuN 和脑源性神经营养因子（BDNF）在 Aβ 注射小鼠海马中的表达。总的来说，我们的结果表明，随着在临床人群中的进一步测试，BJIGT 可能对痴呆症，特别是阿尔茨海默病的治疗具有很大的用途。
