Dept. of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Department of Medical, Surgery and Neurosciences, University of Siena, Siena, Italy.
Mech Ageing Dev. 2018 Jun;172:78-85. doi: 10.1016/j.mad.2017.11.006. Epub 2017 Nov 1.
Scavenger receptor B1 (SR-B1) is a trans-membrane protein, involved in tissue reverse cholesterol transport. Several studies have demonstrated that SR-B1 is also implicated in other physiological processes, such as bacteria and apoptotic cells recognition and regulation of intracellular tocopherol and carotenoids levels. Among the tissues where it is localized, SR-B1 has been shown to be significantly expressed in human epidermis. Our group has demonstrated that SR-B1 levels are down-regulated in human cultured keratinocytes by environmental stressors, such as cigarette smoke, via cellular redox imbalance. Our present study aimed to investigate whether such down-regulation was confirmed in a 3D skin model and under other environmental challengers such as particulate matter and ozone. We also investigated the association between oxidation-induced SR-B1 modulation and impaired wound closure. The data obtained showed that not only cigarette, but also the other environmental stressors reduced SR-B1 expression in epidermal cutaneous tissues and that this effect might be involved in impaired wound healing.
清道夫受体 B1（SR-B1）是一种跨膜蛋白，参与组织逆向胆固醇转运。多项研究表明，SR-B1 还与其他生理过程有关，如细菌和凋亡细胞的识别，以及细胞内维生素 E 和类胡萝卜素水平的调节。在其定位的组织中，已经表明 SR-B1 在人表皮中表达显著。我们的研究小组已经证明，环境应激物，如香烟烟雾，通过细胞氧化还原失衡，下调人培养角质形成细胞中的 SR-B1 水平。本研究旨在探讨这种下调是否在 3D 皮肤模型中得到证实，以及在其他环境挑战物如颗粒物和臭氧下是否得到证实。我们还研究了氧化诱导的 SR-B1 调节与伤口闭合受损之间的关联。获得的数据表明，不仅香烟，而且其他环境应激物也降低了表皮皮肤组织中的 SR-B1 表达，而这种作用可能与伤口愈合受损有关。
