Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 06273, South Korea.
Division of Antimicrobial Resistance Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, South Korea.
Sci Rep. 2024 Oct 21;14(1):24725. doi: 10.1038/s41598-024-73120-5.
Antimicrobials reserved for human medicines are permitted for companion animals and it is important to understand multidrug-resistant pathogens recovered from companion animals in terms of epidemiological correlation with human pathogens and possibility of transmission to human-beings. Seventeen of each CTX-M-type extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) and Klebsiella pneumoniae (ESBL-KP) canine isolates were assessed. Entire genomes of the 34 isolates were sequenced. Plasmid transfer and relative growth rates were assessed at differed temperature conditions indicating the body temperature of dogs, that of human-beings, and environment. ESBL-ECs were clonally diverse, while ESBL-KPs were not. The ESBL-ECs carried the bla gene in plasmids and the bla-like gene either in chromosomes or in plasmids. The ESBL-KPs possessed the bla gene in plasmids (n = 15). One of the isolates carried another bla gene in a chromosome simultaneously and the other isolate had an additional bla gene-harbouring plasmid, together. Two ESBL-KP isolates carried the bla gene in plasmids. Plasmid transfer ESBL-EC to K. pneumoniae was efficient and the differed biological costs by temperature was much more in ESBL-EC than in ESBL-KP. Intersectoral dissemination of ESBL-ECs occurred mainly by horizontal gene transfer, while that of ESBL-KPs occurred by clonal dissemination.
允许将人类医学专用的抗菌药物用于伴侣动物，因此，了解从伴侣动物中分离出的具有多重耐药性的病原体，对于了解其与人类病原体的流行病学相关性以及向人类传播的可能性非常重要。对 17 株产 CTX-M 型扩展谱β-内酰胺酶的大肠杆菌（ESBL-EC）和肺炎克雷伯菌（ESBL-KP）犬分离株进行了评估。对 34 株分离株的全基因组进行了测序。在不同温度条件下评估了质粒转移和相对生长速率，这些温度条件分别代表了犬的体温、人类的体温和环境温度。ESBL-EC 具有克隆多样性，而 ESBL-KP 则没有。ESBL-EC 中的 bla 基因位于质粒上，bla 样基因位于染色体或质粒上。ESBL-KP 携带 bla 基因的质粒（n = 15）。其中一个分离株同时在染色体上携带另一个 bla 基因，另一个分离株则同时携带一个含有 bla 基因的质粒。有两个 ESBL-KP 分离株携带 bla 基因的质粒。ESBL-EC 向肺炎克雷伯菌的质粒转移效率较高，ESBL-EC 的温度相关生物成本比 ESBL-KP 高得多。ESBL-EC 的跨部门传播主要通过水平基因转移发生，而 ESBL-KP 的跨部门传播则通过克隆传播发生。
