Jandak J, Steiner M, Richardson P D
Division of Hematology/Oncology, Brown University, Providence, RI.
Blood. 1989 Jan;73(1):141-9.
Platelet adhesiveness was tested ex vivo in a group of six normal individuals receiving varying doses of alpha-tocopherol. Adhesion to glass slides coated with fibronectin, collagen, fibrinogen, or plasma proteins was studied by perfusing platelet-rich plasma through a flow chamber that allowed time- and space-resolved observations of platelet adhesion. Platelet adherence was measured in an area of parallel flow lines and low shear rate under standardized conditions before and after dietary supplementation with vitamin E at doses of 200 and 400 IU/d. Platelet adherence differed in magnitude depending on the adhesive surface. There was a distinct preference of platelets to adhere to sites that had been previously occupied. A remarkable decrease in platelet adherence was observed after vitamin E supplementation. The average decrease in adhesion after 2 weeks of 200 IU vitamin E was 75%. After 2 weeks of 400 IU vitamin E, platelet adhesion was reduced by 82%. The inhibitory activity of alpha-tocopherol was dose dependent and correlated well with the increase in alpha-tocopherol concentration in platelets after supplementation. Scanning electron microscopy revealed a striking decrease of pseudopodium formation in alpha-tocopherol-enriched platelets. Our results suggest that vitamin E may also be an effective antiadhesive agent in vivo.
在一组六名接受不同剂量α-生育酚的正常个体中进行了体外血小板黏附性测试。通过使富含血小板的血浆灌注通过一个流动腔室来研究血小板与涂有纤连蛋白、胶原蛋白、纤维蛋白原或血浆蛋白的载玻片的黏附情况，该流动腔室允许对血小板黏附进行时间和空间分辨观察。在以200和400 IU/d的剂量补充维生素E前后，在标准化条件下，于平行流线和低剪切速率区域测量血小板黏附情况。血小板黏附的程度因黏附表面而异。血小板明显倾向于黏附在先前已被占据的部位。补充维生素E后观察到血小板黏附显著降低。补充200 IU维生素E两周后，黏附平均降低75%。补充400 IU维生素E两周后，血小板黏附降低82%。α-生育酚的抑制活性呈剂量依赖性，且与补充后血小板中α-生育酚浓度的增加密切相关。扫描电子显微镜显示，富含α-生育酚的血小板中伪足形成显著减少。我们的结果表明，维生素E在体内可能也是一种有效的抗黏附剂。
