Center for CardioVascular and Nutrition Research (C2VN), Aix Marseille Univ, INSERM, INRAE, 13005 Marseille, France.
Department of Science and Technology, Food and Nutrition Research Institute, Bicutan, Taguig City 1631, Philippines.
Nutrients. 2024 Aug 3;16(15):2556. doi: 10.3390/nu16152556.
In humans, α-tocopherol (α-TOC) is mainly stored in adipose tissue, where it participates in preventing damages induced by inflammation and reactive oxygen species. Factors, including genetic ones, that explain adipose tissue α-TOC concentration remain poorly understood. This study, therefore, aimed to characterize the interindividual variability of adipose tissue α-TOC concentration in healthy individuals and to identify single nucleotide polymorphisms (SNPs) associated with it. The study used a randomized cross-over design with 42 healthy adult males. α-TOC concentration was measured in fasting plasma and periumbilical adipose tissue samples, both at fast and 8 h after consumption of three standard meals. Partial least squares (PLS) regression was performed to identify SNPs associated with the interindividual variability of adipose tissue α-TOC concentration. Adipose tissue α-TOC concentration was not associated with fasting plasma concentration (Pearson's = 0.24, 95% CI: [-0.08, 0.51]). There was a high interindividual variability of adipose tissue α-TOC concentration (CV = 61%). A PLS regression model comprising 10 SNPs in five genes (, , , , and ) explained 60% (adjusted ) of the variability of this concentration. The interindividual variability of adipose tissue α-TOC concentration in humans is due, at least partly, to SNPs in genes involved in α-TOC and triglyceride metabolism.
在人体中，α-生育酚（α-TOC）主要储存在脂肪组织中，在脂肪组织中，它参与预防炎症和活性氧物质引起的损伤。解释脂肪组织中 α-TOC 浓度的因素，包括遗传因素，仍知之甚少。因此，本研究旨在描述健康个体脂肪组织中 α-TOC 浓度的个体间变异性，并确定与之相关的单核苷酸多态性（SNP）。该研究采用 42 名健康成年男性的随机交叉设计。在禁食血浆和脐周脂肪组织样本中测量 α-TOC 浓度，在进食三种标准餐后立即和 8 小时分别测量。采用偏最小二乘（PLS）回归来鉴定与脂肪组织 α-TOC 浓度个体间变异性相关的 SNP。脂肪组织 α-TOC 浓度与空腹血浆浓度无关（Pearson's = 0.24，95%CI：[-0.08, 0.51]）。脂肪组织 α-TOC 浓度的个体间变异性很大（CV = 61%）。由五个基因（、、、、和）中的 10 个 SNP 组成的 PLS 回归模型解释了该浓度变异性的 60%（调整）。人类脂肪组织 α-TOC 浓度的个体间变异性至少部分归因于参与 α-TOC 和甘油三酯代谢的基因中的 SNP。
