Ranganathan Abhay, Campo Jessica, Myerson Jacob, Shuvaev Vladimir, Zern Blaine, Muzykantov Vladimir, Eckmann David M
Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 19104, USA.
J Biomed Nanotechnol. 2017 Jun;13(6):737-745. doi: 10.1166/jbn.2017.2392.
Targeted drug delivery is a fast growing industry in healthcare and technologies are being developed for applications utilizing nanocarriers as vehicles for drug transport. As the size scale of these particles becomes further reduced, advanced fluorescence microscopy and image analysis techniques become increasingly important for facilitating our understanding of nanocarrier binding and avidity, thereby establishing the basis for nanocarrier design optimization. While there is a significant body of published work using nanocarriers and , the advent of smaller particles that have typically been studied (~500 nm) limits the ability to attain quantitative measurements of nanocarrier binding dynamics since image acquisition and analysis methods are restricted by microscopy pixel size. This work demonstrates the use of a novel calibration technique based on radioisotope counting and fluorescence imaging for enabling quantitative determination of nanocarrier binding dynamics. The technique is then applied to assess the temporal profile of endothelial cell binding of two antibody targeted nanocarrier types in the presence of fluid shear stress. Results are provided for binding of nanoparticles smaller than a microscopy image pixel.
靶向给药是医疗保健领域中一个快速发展的行业，目前正在开发各种技术，以便将纳米载体用作药物运输的工具。随着这些颗粒的尺寸进一步减小，先进的荧光显微镜和图像分析技术对于促进我们对纳米载体结合和亲和力的理解变得越来越重要，从而为纳米载体设计的优化奠定基础。虽然已经有大量使用纳米载体的已发表作品，但是，由于图像采集和分析方法受到显微镜像素大小的限制，典型研究的较小颗粒（约500 nm）的出现限制了对纳米载体结合动力学进行定量测量的能力。这项工作展示了一种基于放射性同位素计数和荧光成像的新型校准技术的应用，用于定量测定纳米载体的结合动力学。然后将该技术应用于评估在流体剪切应力存在下两种抗体靶向纳米载体类型与内皮细胞结合的时间曲线。给出了小于显微镜图像像素的纳米颗粒的结合结果。
