Zhang Jinbo, Li Shiqing, Sun Li, Chen Yanxia, Zhang Lei, Zhang Zhenghui
Center for Preventive Treatment of Disease, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China.
Department of Encephalopathy, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4453-4459. doi: 10.3892/etm.2017.5092. Epub 2017 Sep 1.
Particulate matter 2.5 (PM) is a growing concern worldwide due to its association with respiratory diseases, including chronic obstructive pulmonary disease (COPD). Stemonine, a traditional Chinese herb, has been demonstrated to exhibit anti-inflammatory and antioxidant properties, making it a potential drug for the treatment of respiratory diseases. The therapeutic effects of stemonine on mice with PM-induced COPD were investigated in the present study. Kunming mice were randomly divided into the following five groups (n=10/group): Control, model, low-dose stemonine, moderate-dose stemonine and high-dose stemonine. The model mice received an intranasal instillation of PM suspension (40 mg/kg). The levels of specific enzymes, markers of oxidative stress, and the inflammatory cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-6 were measured in the bronchoalveolar lavage fluid of the mice using ELISA kits. Hematoxylin and eosin staining was performed to determine inflammatory changes to the lung tissue. It was demonstrated that stemonine could significantly alleviate lung injury by decreasing the levels of enzymes and cytokines associated with inflammation and oxidative stress in a dose-dependent manner. In addition, stemonine dose-dependently increased the amount of superoxide dismutase. These results suggest that stemonine reduces lung inflammation in mice with PM-induced COPD, providing a novel approach for the treatment of PM-induced respiratory diseases.
由于细颗粒物2.5（PM）与包括慢性阻塞性肺疾病（COPD）在内的呼吸道疾病有关，它在全球范围内日益受到关注。百部碱是一种传统中药，已被证明具有抗炎和抗氧化特性，使其成为治疗呼吸道疾病的潜在药物。本研究调查了百部碱对PM诱导的COPD小鼠的治疗效果。将昆明小鼠随机分为以下五组（每组n = 10）：对照组、模型组、低剂量百部碱组、中剂量百部碱组和高剂量百部碱组。模型小鼠经鼻内滴注PM悬浮液（40 mg/kg）。使用ELISA试剂盒测定小鼠支气管肺泡灌洗液中特定酶的水平、氧化应激标志物以及炎性细胞因子肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的水平。进行苏木精和伊红染色以确定肺组织的炎症变化。结果表明，百部碱可以通过以剂量依赖的方式降低与炎症和氧化应激相关的酶和细胞因子水平，显著减轻肺损伤。此外，百部碱剂量依赖性地增加超氧化物歧化酶的量。这些结果表明，百部碱可减轻PM诱导的COPD小鼠的肺部炎症反应，为治疗PM诱导的呼吸道疾病提供了一种新方法。
