Department of Urology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).
Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).
Med Sci Monit. 2018 Jul 12;24:4807-4822. doi: 10.12659/MSM.908057.
BACKGROUND microRNAs (miRNAs) have a role as biomarkers in human cancer. The aim of this study was to use bioinformatics data, and review of cases identified from the literature, to investigate the role of microRNA-99a-3p (miR-99a-3p) in prostate cancer, including the identification of its target genes and signaling pathways. MATERIAL AND METHODS Meta-analysis from a literature review included 965 cases of prostate cancer. Bioinformatics databases interrogated for miR-99a-3p in prostate cancer included The Cancer Genome Atlas (TCGA), the Gene Expression Omnibus (GEO), and ArrayExpress. Twelve computational predictive algorithms were developed to integrate miR-99a-3p target gene prediction data. Bioinformatics analysis data from Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) network analysis were used investigate the possible pathways and target genes for miR-99a-3p in prostate cancer. RESULTS TCGA data showed that miR-99a was down-regulated in prostate cancer when compared with normal prostate tissue. Receiver-operating characteristic (ROC) curve area under the curve (AUC) for miR-99a-3p was 0.660 (95% CI, 0.587-0.732) or a moderate level of discriminations. Pathway analysis showed that miR-99a-3p was associated with the Wnt and vascular endothelial growth factor (VEGF) signaling pathways. The PPP3CA and HYOU1 genes, selected from the PPI network, were highly expressed in prostate cancer tissue compared with normal prostate tissue, and negatively correlated with the expression of miR-99a-3p. CONCLUSIONS In prostate cancer, miR-99a-3p expression was associated with the Wnt and VEGF signaling pathways, which might inhibit the expression of PPP3CA or HYOU1.
微小 RNA（miRNAs）在人类癌症中作为生物标志物发挥作用。本研究的目的是利用生物信息学数据和文献综述中确定的病例回顾，研究 microRNA-99a-3p（miR-99a-3p）在前列腺癌中的作用，包括鉴定其靶基因和信号通路。
文献综述的荟萃分析纳入了 965 例前列腺癌病例。用于前列腺癌中 miR-99a-3p 分析的生物信息学数据库包括癌症基因组图谱（TCGA）、基因表达综合（GEO）和 ArrayExpress。开发了 12 种计算预测算法来整合 miR-99a-3p 靶基因预测数据。使用基因本体论（GO）、京都基因与基因组百科全书（KEGG）和蛋白质-蛋白质相互作用（PPI）网络分析的生物信息学分析数据来研究 miR-99a-3p 在前列腺癌中的可能通路和靶基因。
TCGA 数据显示，与正常前列腺组织相比，前列腺癌中 miR-99a 下调。miR-99a-3p 的接收器工作特征（ROC）曲线下面积（AUC）为 0.660（95%CI，0.587-0.732），为中等水平的区分度。通路分析表明，miR-99a-3p 与 Wnt 和血管内皮生长因子（VEGF）信号通路相关。从 PPI 网络中选择的 PPP3CA 和 HYOU1 基因在前列腺癌组织中的表达高于正常前列腺组织，且与 miR-99a-3p 的表达呈负相关。
在前列腺癌中，miR-99a-3p 的表达与 Wnt 和 VEGF 信号通路相关，这可能抑制 PPP3CA 或 HYOU1 的表达。
